Active holographic tomograph for measuring biological microbodies

Arkadiusz Kuś

Abstract

The thesis is based on a series of ten publications in reviewed journals eight of which are on JCR list and the remaining two are published in reviewed conference materials. The enclosed guide to the publications describes the research performed during the preparation of the PhD thesis. The topic of this work is the development of holographic tomography systems designed for biological samples. The proposed systems exhibit increased measurement range and offer improved reconstruction accuracy. There are two types of systems described in the thesis: tomography with object rotation, which provides full projection angle, and with illumination scanning, which exhibits limited projection angle. The first type of the built measurement systems used rotation of the object placed inside a capillary. The simplified design of the optical setup was successfully used to retrieve 3D refractive index distribution of live cells and 3D phase phantoms. The results of these measurements were later used as reference data for testing algorithms in limited-angle optical diffraction tomography. The main focus in this work was the development and enhancements of the limited-angle tomography systems. The proposed measurement systems provide extended measurement range, enable more illumination scenarios and offer increased depth of field and improved reconstruction accuracy. The optical system was built as a Mach-Zehnder-interferometer-based holographic microscope with a custom-built optical system in the reference beam, which introduced the flexibility in choosing a sample illumination scenario, and with electrically-driven defocusing lens in imaging module. A thorough analysis of the influence of illumination optical system and scanning errors on the reconstruction quality was performed. By replacing mechanical scanning with a liquid crystal spatial light modulator (LC SLM) and actively correcting the systematic errors of the illumination wavefront, the accuracy of the reconstruction was improved. Finally, a new type of objects as calibration phantoms for holographic tomography was verified. The developed systems were also applied to study human cancer cells and mouse fibroblasts and myoblasts.
Diploma typeDoctor of Philosophy
Author Arkadiusz Kuś (FM / IMPh)
Arkadiusz Kuś,,
- The Institute of Micromechanics and Photonics
Title in EnglishActive holographic tomograph for measuring biological microbodies
Languagepl polski
Certifying UnitFaculty of Mechatronics (FM)
Disciplinemechanical engineering / (technology domain) / (technological sciences)
Start date01-11-2012
Defense Date13-12-2017
End date20-12-2017
Supervisor Małgorzata Kujawińska (FM / IMPh)
Małgorzata Kujawińska,,
- The Institute of Micromechanics and Photonics

Michał Józwik (FM / IMPh)
Michał Józwik,,
- The Institute of Micromechanics and Photonics

Honoredyes
Pages165
Keywords in Englishoptics, digital holographic microscopy, tomography, biological microstructures, optical design
Abstract in EnglishThe thesis is based on a series of ten publications in reviewed journals eight of which are on JCR list and the remaining two are published in reviewed conference materials. The enclosed guide to the publications describes the research performed during the preparation of the PhD thesis. The topic of this work is the development of holographic tomography systems designed for biological samples. The proposed systems exhibit increased measurement range and offer improved reconstruction accuracy. There are two types of systems described in the thesis: tomography with object rotation, which provides full projection angle, and with illumination scanning, which exhibits limited projection angle. The first type of the built measurement systems used rotation of the object placed inside a capillary. The simplified design of the optical setup was successfully used to retrieve 3D refractive index distribution of live cells and 3D phase phantoms. The results of these measurements were later used as reference data for testing algorithms in limited-angle optical diffraction tomography. The main focus in this work was the development and enhancements of the limited-angle tomography systems. The proposed measurement systems provide extended measurement range, enable more illumination scenarios and offer increased depth of field and improved reconstruction accuracy. The optical system was built as a Mach-Zehnder-interferometer-based holographic microscope with a custom-built optical system in the reference beam, which introduced the flexibility in choosing a sample illumination scenario, and with electrically-driven defocusing lens in imaging module. A thorough analysis of the influence of illumination optical system and scanning errors on the reconstruction quality was performed. By replacing mechanical scanning with a liquid crystal spatial light modulator (LC SLM) and actively correcting the systematic errors of the illumination wavefront, the accuracy of the reconstruction was improved. Finally, a new type of objects as calibration phantoms for holographic tomography was verified. The developed systems were also applied to study human cancer cells and mouse fibroblasts and myoblasts.
Thesis file
RozprawaAK-wydruk-2017-10-18.pdf of 04-12-2017
57.94 MB

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