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Active holographic tomograph for measuring biological microbodies

Arkadiusz Kuś

Abstract

The thesis is based on a series of ten publications in reviewed journals eight of which are on JCR list and the remaining two are published in reviewed conference materials. The enclosed guide to the publications describes the research performed during the preparation of the PhD thesis. The topic of this work is the development of holographic tomography systems designed for biological samples. The proposed systems exhibit increased measurement range and offer improved reconstruction accuracy. There are two types of systems described in the thesis: tomography with object rotation, which provides full projection angle, and with illumination scanning, which exhibits limited projection angle. The first type of the built measurement systems used rotation of the object placed inside a capillary. The simplified design of the optical setup was successfully used to retrieve 3D refractive index distribution of live cells and 3D phase phantoms. The results of these measurements were later used as reference data for testing algorithms in limited-angle optical diffraction tomography. The main focus in this work was the development and enhancements of the limited-angle tomography systems. The proposed measurement systems provide extended measurement range, enable more illumination scenarios and offer increased depth of field and improved reconstruction accuracy. The optical system was built as a Mach-Zehnder-interferometer-based holographic microscope with a custom-built optical system in the reference beam, which introduced the flexibility in choosing a sample illumination scenario, and with electrically-driven defocusing lens in imaging module. A thorough analysis of the influence of illumination optical system and scanning errors on the reconstruction quality was performed. By replacing mechanical scanning with a liquid crystal spatial light modulator (LC SLM) and actively correcting the systematic errors of the illumination wavefront, the accuracy of the reconstruction was improved. Finally, a new type of objects as calibration phantoms for holographic tomography was verified. The developed systems were also applied to study human cancer cells and mouse fibroblasts and myoblasts.
Record ID
WUTfa12a83e47e848dfac31ade082c1b5cc
Diploma type
Doctor of Philosophy
Author
Title in Polish
Aktywny tomograf holograficzny do pomiaru mikroobiektów biologicznych
Title in English
Active holographic tomograph for measuring biological microbodies
Language
(pl) Polish
Certifying Unit
Faculty of Mechatronics (FM)
Discipline
mechanical engineering / (technology domain) / (technological sciences)
Status
Finished
Start date
01-11-2012
Defense Date
13-12-2017
Title date
20-12-2017
Supervisor
Honored
yes
Pages
165
Keywords in English
optics, digital holographic microscopy, tomography, biological microstructures, optical design
Abstract in English
The thesis is based on a series of ten publications in reviewed journals eight of which are on JCR list and the remaining two are published in reviewed conference materials. The enclosed guide to the publications describes the research performed during the preparation of the PhD thesis. The topic of this work is the development of holographic tomography systems designed for biological samples. The proposed systems exhibit increased measurement range and offer improved reconstruction accuracy. There are two types of systems described in the thesis: tomography with object rotation, which provides full projection angle, and with illumination scanning, which exhibits limited projection angle. The first type of the built measurement systems used rotation of the object placed inside a capillary. The simplified design of the optical setup was successfully used to retrieve 3D refractive index distribution of live cells and 3D phase phantoms. The results of these measurements were later used as reference data for testing algorithms in limited-angle optical diffraction tomography. The main focus in this work was the development and enhancements of the limited-angle tomography systems. The proposed measurement systems provide extended measurement range, enable more illumination scenarios and offer increased depth of field and improved reconstruction accuracy. The optical system was built as a Mach-Zehnder-interferometer-based holographic microscope with a custom-built optical system in the reference beam, which introduced the flexibility in choosing a sample illumination scenario, and with electrically-driven defocusing lens in imaging module. A thorough analysis of the influence of illumination optical system and scanning errors on the reconstruction quality was performed. By replacing mechanical scanning with a liquid crystal spatial light modulator (LC SLM) and actively correcting the systematic errors of the illumination wavefront, the accuracy of the reconstruction was improved. Finally, a new type of objects as calibration phantoms for holographic tomography was verified. The developed systems were also applied to study human cancer cells and mouse fibroblasts and myoblasts.
Thesis file
  • File: 1
    RozprawaAK-wydruk-2017-10-18.pdf
    of 04-12-2017
    57 MB
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Uniform Resource Identifier
https://repo.pw.edu.pl/info/phd/WUTfa12a83e47e848dfac31ade082c1b5cc/
URN
urn:pw-repo:WUTfa12a83e47e848dfac31ade082c1b5cc

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