Zastosowanie Clostridium cellulolyticum do intensyfikacji fazy hydrolizy podczas fermentacji metanowej odpadów ulegających biodegradacji

Jarosław Turek

Abstract

Two types of waste, 20-80 mm fraction of municipal solid waste and green waste, were subjected to dry fermentation. Clostridium cellulolyticum, cultured in anaerobic conditions in 35 ºC for 48h, was added to the fermentation mass. The effectiveness of culturing was measured to be 1,4x106 cells per 1 ml. The bioaugumentation dosage ranged from 5 to 125 µl, corresponding to cell number ranging from 7000 to 175000 cells per 1 g of waste. The addition of 5 µl of Clostridium cellulolyticum per 1 g of 20-80 mm fraction of municipal solid waste had no effect on hydrolysis stage of fermentation. Increase of C. cellulolyticum in fermentation mass to 125 µl per 1 g of waste resulted in 3 fold increased concentration of Volatile Fatty Acids (VFA) in the bioaugumented samples. Introduction of the Clostridium cellulolyticum into more homogenous environment such as green waste resulted in rise of gas production during dry fermentation form 18 to 29 dm3 /kg d.o.m. DGGE method was used to document the changes in the biodiversity during the first stage of fermentation and the effects of bioaugumentation, including the presence of introduced into fermentation mass cellulolytic bacteria. Both 20-80 mm fraction of municipal solid waste and green waste showed high biodiversity level, represented by at least 30 amplicons in each sample.
Diploma typeDoctor of Philosophy
Author Jarosław Turek (FEE)
Jarosław Turek,,
- Faculty of Environmental Engineering
Title in PolishZastosowanie Clostridium cellulolyticum do intensyfikacji fazy hydrolizy podczas fermentacji metanowej odpadów ulegających biodegradacji
Languagepl polski
Certifying UnitFaculty of Environmental Engineering (FEE)
Disciplineenvironmental engineering / (technology domain) / (technological sciences)
Defense Date01-06-2010
End date15-06-2010
Supervisor Irena Wojnowska-Bryła (FEE)
Irena Wojnowska-Bryła,,
- Faculty of Environmental Engineering

Internal reviewers Maria Łebkowska (FEE / DB)
Maria Łebkowska,,
- Department of Biology
External reviewers Jacek Bielecki
Jacek Bielecki,,
-
Pages114
Keywords in Englishxxx
Abstract in EnglishTwo types of waste, 20-80 mm fraction of municipal solid waste and green waste, were subjected to dry fermentation. Clostridium cellulolyticum, cultured in anaerobic conditions in 35 ºC for 48h, was added to the fermentation mass. The effectiveness of culturing was measured to be 1,4x106 cells per 1 ml. The bioaugumentation dosage ranged from 5 to 125 µl, corresponding to cell number ranging from 7000 to 175000 cells per 1 g of waste. The addition of 5 µl of Clostridium cellulolyticum per 1 g of 20-80 mm fraction of municipal solid waste had no effect on hydrolysis stage of fermentation. Increase of C. cellulolyticum in fermentation mass to 125 µl per 1 g of waste resulted in 3 fold increased concentration of Volatile Fatty Acids (VFA) in the bioaugumented samples. Introduction of the Clostridium cellulolyticum into more homogenous environment such as green waste resulted in rise of gas production during dry fermentation form 18 to 29 dm3 /kg d.o.m. DGGE method was used to document the changes in the biodiversity during the first stage of fermentation and the effects of bioaugumentation, including the presence of introduced into fermentation mass cellulolytic bacteria. Both 20-80 mm fraction of municipal solid waste and green waste showed high biodiversity level, represented by at least 30 amplicons in each sample.
Thesis file
Turek.pdf 2.92 MB
Citation count*5 (2020-09-26)

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