Badania nad miniaturyzacją systemów analitycznych stosowanych w proteomice

Alicja Filipowicz-Szymańska

Abstract

The Ph.D. thesis entitled Research on miniaturization of analytical systems for proteomics presents the design, fabrication process and tests of analytical microsystem dedicated to proteomics research. Miniaturization of two steps of proteomic research is presented: enzymatic hydrolysis of proteins and separation of its products. In the literature part of this PhD thesis the concept of miniaturization and its application in proteomic research is described. Further part is devoted to enzymatic microreactors and their applications. In the next chapter, monolithic phases and their use in microtechnology is presented. The experimental part is divided into two chapters. In the first one a research on enzymatic microreactors is presented. The design and the fabrication process of a microreactor with free trypsin is described. Furthermore, two methods of enzyme immobilization in microsystems are shown. The first one employs the encapsulation of the enzyme in alginate particles generated by the electrostatic droplet generation method and introduction the beads into the microreactor. Covalent technique is the second method of enzyme immobilization in microsystems. The enzyme was immobilized on silver-coated microchannel. The activity tests of generated enzymatic microreactors are presented. In the second part of the experimental section, a microsystem for the injection, chromatographic separation and detection is described. The research focused on the fabrication of monolithic column in the microscale. The method of pressure pinched injection of probe onto monolithic column was developed. In the next part the study on the separation of dyes and proteins is presented. Concluding remarks are placed in the last part of the Ph.D. thesis.
Diploma typeDoctor of Philosophy
Author Alicja Filipowicz-Szymańska (FC)
Alicja Filipowicz-Szymańska,,
- Faculty of Chemistry
Title in PolishBadania nad miniaturyzacją systemów analitycznych stosowanych w proteomice
Languagepl polski
Certifying UnitFaculty of Chemistry (FC)
Disciplinechemical technology / (technology domain) / (technological sciences)
Defense Date18-01-2010
End date23-02-2010
Supervisor Zbigniew Brzózka (FC / IBC / DM)
Zbigniew Brzózka,,
- Department Of Microbioanalytics

Internal reviewers Maria Balcerzak (FC / CAC)
Maria Balcerzak,,
- Chair Of Analytical Chemistry
External reviewers Bogusław Buszewski
Bogusław Buszewski,,
-
Pages156
Keywords in Englishxxx
Abstract in EnglishThe Ph.D. thesis entitled Research on miniaturization of analytical systems for proteomics presents the design, fabrication process and tests of analytical microsystem dedicated to proteomics research. Miniaturization of two steps of proteomic research is presented: enzymatic hydrolysis of proteins and separation of its products. In the literature part of this PhD thesis the concept of miniaturization and its application in proteomic research is described. Further part is devoted to enzymatic microreactors and their applications. In the next chapter, monolithic phases and their use in microtechnology is presented. The experimental part is divided into two chapters. In the first one a research on enzymatic microreactors is presented. The design and the fabrication process of a microreactor with free trypsin is described. Furthermore, two methods of enzyme immobilization in microsystems are shown. The first one employs the encapsulation of the enzyme in alginate particles generated by the electrostatic droplet generation method and introduction the beads into the microreactor. Covalent technique is the second method of enzyme immobilization in microsystems. The enzyme was immobilized on silver-coated microchannel. The activity tests of generated enzymatic microreactors are presented. In the second part of the experimental section, a microsystem for the injection, chromatographic separation and detection is described. The research focused on the fabrication of monolithic column in the microscale. The method of pressure pinched injection of probe onto monolithic column was developed. In the next part the study on the separation of dyes and proteins is presented. Concluding remarks are placed in the last part of the Ph.D. thesis.
Thesis file
Filipowicz-Szymanska.pdf 8.28 MB
Citation count*5 (2020-09-18)

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