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Bioługowanie metali ciężkich z odpadów paleniskowych wspomagane mikroorganizmami wytwarzającymi biosurfaktanty

Dorota Andrzejewska-Morzuch

Abstract

The aim of the research was to elaborate the effective method of heavy metal removal from combustion wastes using bioleaching process based on mixed auto- and heterotrophic bacterial consortia, including biosurfactant-producing microorganisms. Chosen combustion wastes: ashes and slags were characterized in respect of their grain size, water content as well as concentration and speciation of metals: Zn, Cu, Pb, Ni, Cd i Cr. The bioleaching culture of active strains was developed. Then, using the culture, the susceptibility of combustion wastes (obtained from different sources) to bioleaching process was determined. The influence of grain size, water content, metals speciation as well as process parameters: temperature, mixing intensity and aeration on bioleaching effectiveness was taken into consideration. The research was carried out both in laboratory and technical fractional scale. It was revealed, that combustion wastes differ in respect of heavy metals conentration and their speciation, grain size and water content. The highest effectiveness of the metal release was observed for the sewage sludge – based bioleaching culture containing both sulpur oxidizing bacteria and biosurfactant producing microorganisms. Biosurfactant producers were able to survive in the proces sof metals bioleaching from combustion wastes. The particular combustion wastes revealed the different susceptibility to bioleaching process in developed bioleaching medium. The highest effectiveness of metals removal was observed for the slag Z. The largest amounts of metals were released from wastes of grain size about 0,7-1,0mm. The influence of water content in the wastes on bioleaching effectiveness was not observed. The temeprature 22oC and aeration of the culture were parameters influencing positively the bioleaching process, which was proved by metabolic activity tests. The PCR and FISH analysis confirmed the presence of sulphur oxidizing bacteria Acidithiobacillus thiooxidans in bioleaching culture. Among heterotrophic microorganisms Bacillus cereus and Shewanella putrefaciens (in the ash) and Burkholderia cepacia, Bacillus cereus., Delftia acidovorans and Pseudomonas fluorescens (in the slag) predominated. The positive influence of biosurfactant producing bacteria on heavy metals release from combustion wastes was confirmed in flow-column tests and in technical fractional scale. In dynamic conditions, the presence of biosurfactant producing microorganisms resulted in the increase of process effectiveness for copper, chromium zinc and nickel. In bioreactor about 90% of Zn, Cu and Ni as well as 70% of Pb and more than 60% of Cr was bioleached. The research revealed that the process of the metals removal from combustion wastes may be carreid out not only in acidic environment but also in neutral pH, with the presence of biosurfactant producing bacteria as a metal release stimulating factor.
Record ID
WUT246928b1174a4e928865130baa365da6
Diploma type
Doctor of Philosophy
Author
Dorota Andrzejewska-Morzuch Dorota Andrzejewska-Morzuch,, Undefined Affiliation
Title in Polish
Bioługowanie metali ciężkich z odpadów paleniskowych wspomagane mikroorganizmami wytwarzającymi biosurfaktanty
Language
(pl) Polish
Certifying Unit
Faculty of Environmental Engineering (FEE)
Discipline
environmental engineering / (technology domain) / (technological sciences)
Status
Finished
Defense Date
08-04-2014
Supervisor
Internal reviewers
Maria Łebkowska Maria Łebkowska,, Department of Biology (FEE/DB)Faculty of Building Services, Hydro and Environmental Engineering (FEE)
External reviewers
L Kalisz L Kalisz,, Undefined Affiliation
Pages
96
Keywords in English
XXX
Abstract in English
The aim of the research was to elaborate the effective method of heavy metal removal from combustion wastes using bioleaching process based on mixed auto- and heterotrophic bacterial consortia, including biosurfactant-producing microorganisms. Chosen combustion wastes: ashes and slags were characterized in respect of their grain size, water content as well as concentration and speciation of metals: Zn, Cu, Pb, Ni, Cd i Cr. The bioleaching culture of active strains was developed. Then, using the culture, the susceptibility of combustion wastes (obtained from different sources) to bioleaching process was determined. The influence of grain size, water content, metals speciation as well as process parameters: temperature, mixing intensity and aeration on bioleaching effectiveness was taken into consideration. The research was carried out both in laboratory and technical fractional scale. It was revealed, that combustion wastes differ in respect of heavy metals conentration and their speciation, grain size and water content. The highest effectiveness of the metal release was observed for the sewage sludge – based bioleaching culture containing both sulpur oxidizing bacteria and biosurfactant producing microorganisms. Biosurfactant producers were able to survive in the proces sof metals bioleaching from combustion wastes. The particular combustion wastes revealed the different susceptibility to bioleaching process in developed bioleaching medium. The highest effectiveness of metals removal was observed for the slag Z. The largest amounts of metals were released from wastes of grain size about 0,7-1,0mm. The influence of water content in the wastes on bioleaching effectiveness was not observed. The temeprature 22oC and aeration of the culture were parameters influencing positively the bioleaching process, which was proved by metabolic activity tests. The PCR and FISH analysis confirmed the presence of sulphur oxidizing bacteria Acidithiobacillus thiooxidans in bioleaching culture. Among heterotrophic microorganisms Bacillus cereus and Shewanella putrefaciens (in the ash) and Burkholderia cepacia, Bacillus cereus., Delftia acidovorans and Pseudomonas fluorescens (in the slag) predominated. The positive influence of biosurfactant producing bacteria on heavy metals release from combustion wastes was confirmed in flow-column tests and in technical fractional scale. In dynamic conditions, the presence of biosurfactant producing microorganisms resulted in the increase of process effectiveness for copper, chromium zinc and nickel. In bioreactor about 90% of Zn, Cu and Ni as well as 70% of Pb and more than 60% of Cr was bioleached. The research revealed that the process of the metals removal from combustion wastes may be carreid out not only in acidic environment but also in neutral pH, with the presence of biosurfactant producing bacteria as a metal release stimulating factor.
Thesis file
  • File: 1
    Andrzejewska Dorota.pdf
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Citation count
2

Uniform Resource Identifier
https://repo.pw.edu.pl/info/phd/WUT246928b1174a4e928865130baa365da6/
URN
urn:pw-repo:WUT246928b1174a4e928865130baa365da6

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