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ANALYSIS OF ASPERIGILLUS NIGER CULTURES SAMPLES USING HYBRID MINIATURIZED ELECTRONIC TONGUE

Sylwia Małgorzata Dziedzic-Talipska

Abstract

The thesis presents the analysis of selected biological samples with a miniaturized electronic tongue. In order to obtain samples, two cultures of the fungus Asperigillus niger were carried out for citric acid production. The first culture was conducted on a synthetic substrate based on sucrose, whereas the major component of the other culture medium was beet molasses. Both cultures were carried out for two weeks at 30 ˚C, in shaking conditions. Sampling was performed every day at the same time. The developed hybrid electronic tongue (ET) was based on potentiometric, voltammetric, amperometric, conductometric and pH measurements. The role of pattern recognition block was played by Principal Component Analysis and Partial Least Squares Analysis. The first step of the presented work, was the design and the preparation of miniaturized potentiometric ion selective electrodes. For optimization purposes their work parameters were studied under flow conditions using a peristaltic pump and syringe pump. For measurements of the cultured samples electrodes selective towards Na+, H+, CH3COO- ions, lipophilic anions and lipophilic cations were used. During voltammetric measurements a cell consisting of three electrodes was used: a gold working electrode, a platinum auxiliary electrode and reference silver/silver chloride electrode. Cyclic Voltammetry, Differential Pulse and Square Wave Voltammetry were applied for the characterization of the samples. For amperometric measurements the glucose biosensor was used, whereas pH and conductometric measurements were performed using conventional electrodes. The total acidity of the medium as well as the samples from the final stage of the culture was studied in order to estimate the quantity of citric acid. Chemometric analysis results indicated, that using a hybrid electronic tongue, samples from different stages of breeding can be differentiated and therefore the device can be used in fermentation monitoring of citric acid biosynthesis. Furthermore, it was noted that the hybrid ET can distinguish samples from breeding conducted on different substrates
Record ID
WUT4dbe5f178dc0403495d702752f33758b
Diploma type
Master of Science
Author
Sylwia Małgorzata Dziedzic-Talipska Sylwia Małgorzata Dziedzic-Talipska,, Undefined Affiliation
Title in Polish
ANALIZA PRÓBEK POCHODZĄCYCH Z HODOWLI ASPERIGILLUS NIGER ZA POMOCĄ HYBRYDOWEGO ZMINIATURYZOWANEGO ELEKTRONICZNEGO JĘZYKA
Supervisor
Patrycja Ciosek (FC/CMB) Patrycja Ciosek,, Chair of Medical Biotechnology (FC/CMB)Faculty of Chemistry (FC)
Certifying unit
Faculty of Chemistry (FC)
Affiliation unit
Department Of Microbioanalytics (FC/CMB)
Study subject / specialization
, Mikrobioanalityka
Language
(pl) Polish
Status
Finished
Defense Date
10-07-2012
Issue date (year)
2012
Keywords in Polish
-
Keywords in English
-
Abstract in Polish
urn:pw-repo:WUT4dbe5f178dc0403495d702752f33758b