Joint forces of direct, single particle, CE- and HPLC-inductively coupled plasma mass spectrometry techniques for the examination of gold nanoparticle accumulation, distribution and changes inside human cells

Joanna Kruszewska (Legat) , Dominika Kulpińska , Magdalena Matczuk , Ilona Grabowska-Jadach

Abstract

The intracellular localization and transformation of gold nanoparticles (AuNPs) are among the crucial aspects in future applications in cancer therapy. In the context of the study, inductively coupled plasma mass spectrometry (ICP-MS)-based techniques were effectively applied to reveal the fate of AuNPs internalized in cancerous MCF-7 cells. Direct ICP-MS was used to obtain quantitative information about the distribution rate of gold from the AuNPs in the cells, namely their membranes, cytosol as well as nuclei. Moreover, the combination of capillary electrophoresis and reversed-phase liquid chromatography with ICP-MS was used as a tool to probe and compare for the effective monitoring of the speciation changes of the gold-containing forms in the cytosol. The chemical nature (ionic vs. nano) of the metal detected in the cytosol was verified via ICP-MS in a single-particle mode, confirming the stability of the nanomaterials and the absence of ionic gold forms inside the cells.

Author Joanna Kruszewska (Legat) (FC / CAC)
Joanna Kruszewska (Legat),,
- Chair Of Analytical Chemistry
, Dominika Kulpińska (FC / CMB)
Dominika Kulpińska,,
- Chair of Medical Biotechnology
, Magdalena Matczuk (FC / CAC)
Magdalena Matczuk,,
- Chair Of Analytical Chemistry
, Ilona Grabowska-Jadach (FC / CMB)
Ilona Grabowska-Jadach,,
- Chair of Medical Biotechnology
Journal seriesMetallomics, [METALLOMICS], ISSN 1756-5901, e-ISSN 1756-591X
Issue year2020
Vol12
Pages408 - 415
Publication size in sheets0.5
ASJC Classification1303 Biochemistry; 1304 Biophysics; 1601 Chemistry (miscellaneous); 2502 Biomaterials; 2506 Metals and Alloys
DOIDOI:10.1039/c9mt00309f
URL https://pubs.rsc.org/en/content/articlelanding/2020/mt/c9mt00309f#!divAbstract
Languageen angielski
File
c9mt00309f.pdf 2.65 MB
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 07-08-2020, ArticleFromJournal
Publication indicators Scopus Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.073; WoS Impact Factor: 2018 = 3.571 (2) - 2018=3.972 (5)
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