Revisiting catechol derivatives as robust chromogenic hydrogen donors working in alkaline media for peroxidase mimetics

Marcin Drozd , Mariusz Pietrzak , Jakub Pytlos , Elżbieta Malinowska

Abstract

Colloidal noble metal-based nanoparticles are able to catalyze oxidation of chromogenic substrates by H2O2, similarly to peroxidases, even in basic media. However, lack of robust chromogens, which work in high pH impedes their real applications. Herein we demonstrate the applicability of selected catechol derivatives: bromopyrogallol red (BPR) and pyrogallol (PG) as chromogenic substrates for peroxidase-like activity assays, which are capable of working over wide range of pH, covering also basic values. Hyperbranched polyglycidol-stabilized gold nanoparticles (HBPG@AuNPs) were used as model enzyme mimetics. Efficiency of several methods of improving stability of substrates in alkaline media by means of selective suppression of their autoxidation by molecular oxygen was evaluated. In a framework of presented studies the impact of borate anion, applied as complexing agent for PG and BPR, on their stability and reactivity towards oxidation mediated by catalytic AuNPs was investigated. The key role of high concentration of hydrogen peroxide in elimination of non-catalytic oxidation of PG and improvement of optical properties of BPR in alkaline media containing borate was underlined. Described methods of peroxidase-like activity characterization with the use of BPR and PG can become universal tools for characterization of nanozymes, which gain various applications, among others, they are used as catalytic labels in bioassays and biosensors.
Author Marcin Drozd ZMB
Marcin Drozd,,
- Department Of Microbioanalytics
, Mariusz Pietrzak ZMB
Mariusz Pietrzak,,
- Department Of Microbioanalytics
, Jakub Pytlos ZMB
Jakub Pytlos,,
- Department Of Microbioanalytics
, Elżbieta Malinowska ZMB
Elżbieta Malinowska,,
- Department Of Microbioanalytics
Journal seriesAnalytica Chimica Acta, ISSN 0003-2670
Issue year2016
Vol948
Pages80-89
Publication size in sheets0.5
Keywords in EnglishCatalytic activity; Gold nanoparticles; Peroxidase; Pyrogallol; Bromopyrogallol red; Borate complexes
DOIDOI:10.1016/j.aca.2016.10.027
URL http://www.sciencedirect.com/science/article/pii/S0003267016312399
Languageen angielski
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Score (nominal)45
ScoreMinisterial score = 45.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 45.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 4.95 (2) - 2016=4.849 (5)
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