Genotoxicity assessment of aluminium oxide nanoparticles in relation to Escherichia coli and Aeromonas hydrophila
Nina Doskocz , Monika Załęska-Radziwiłł , Katarzyna Anna Affek
AbstractIncreasing production and use of nanoparticles contributes to their widespread dissemination in the environment and their unique physical and chemical properties lead to unlimited distribution in environmental compartments. Migration ability of nanoparticles can have very dangerous consequences, as they can be transferred to potable water. Genotoxicity biomarkers are regarded as useful tools for the assessment of chemical hazards in aquatic ecosystems, because chemicals which damage DNA can significantly alter the functioning of ecosystems. Recent advances in molecular biology have led to the development of several techniques, which can be used for DNA analysis in the field of genetic ecotoxicology. The randomly amplified polymorphic DNA (RAPD) method is a PCR-based technique that amplifies random DNA fragments with the use of single short primers of arbitrary nucleotide sequence under low annealing conditions. RAPD-PCR test has been used successfully for detection of genetic damage in animals and plants. The SOS-Chromotest is one of the most commonly used bacterial tests. It is based on the induction, by genotoxic compounds, of a bacterial SOS repair system conjugated to the b-galactosidase gene and the subsequent measurement of the enzyme expression. In this work, genotoxicity studies on the basis of the RAPD-PCR and SOS-Chromotest assay were performed for aluminium oxide nanoparticles (nano-Al2O3). Little information is available on the fate, transport, and effects of nanomaterials, including metal based particles such as nano-Al2O3, in the environment. The interest in nano-Al2O3 is due to the fact that their influence on genetic material of bacteria is practically unknown. Results obtained for the nanocompound were compared with those for Al2O3 macro form. The nanocompound caused changes in the genetic material of bacteria A. hydrophila. Degree of genetic similarity of obtained profiles bands for primer OPA2 differed from the results obtained for the negative control by more than 27,3%, while from positive control - only by 15,6%. Furthermore, the largest decrease in genetic stability was 89,3%. The values of genotoxicity induction coefficient (I) in the SOS-Chromotest showed strong genotoxicity for nano-Al2O3, in the presence of S9 fraction and slight genotoxicity in the absence of S9 fraction in mutants of Escherichia coli. The results showed also that the nano-Al2O3 can induce genotoxicity a greater extent than the same compounds in their macro form.
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SETAC Europe 28th Annual Meeting.Responsible and Innovative Research for Environmental Quality: Abstract Book, Society of Environmental Toxicology and Chemistry Europe (SETAC Europe), 2018, Rzym, Society of Environmental Toxicology and Chemistry Europe, 536 p.
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