Improving osteoblasts cells proliferation via femtosecond laser surface modification of 3D-printed poly-ε-caprolactone scaffolds for bone tissue engineering applications

A. Daskalova , Barbara Ostrowska , A. Zhelyazkova , Wojciech Święszkowski , A. Trifonov , H. Declercq , C.S.R. Nathala , Karol Szlązak , Maciej Łojkowski , W. Husinsky , I. Buchvarov

Abstract

Synthetic polymer biomaterials incorporating cells are a promising technique for treatment of orthopedic injuries. To enhance the integration of biomaterials into the human body, additional functionalization of the scaffold surface should be carried out that would assist one in mimicking the natural cellular environment. In this study, we examined poly-ɛ-caprolactone (PCL) fiber matrices in view of optimizing the porous properties of the constructs. Altering the porosity of a PCL scaffold is expected to improve the material's biocompatibility, thus influencing its osteoconductivity and osteointegration. We produced 3D poly-ɛ-caprolactone (PCL) matrices by a fused deposition modeling method for bone and cartilage tissue engineering and performed femtosecond (fs) laser modification experiments to improve the surface properties of the PCL construct. Femtosecond laser processing is one of the useful tools for creating a vast diversity of surface patterns with reproducibility and precision. The processed surface of the PCL matrix was examined to follow the effect of the laser parameters, namely the laser pulse energy and repetition rate and the number ( N) of applied pulses. The modified zones were characterized by scanning electron microscopy (SEM), confocal microscopy, X-ray computed tomography and contact angle measurements. The results obtained demonstrated changes in the morphology of the processed surface. A decrease in the water contact angle was also seen after fs laser processing of fiber meshes. Our work demonstrated that a precise control of material surface properties could be achieved by applying a different number of laser pulses at various laser fluence values. We concluded that the structural features of the matrix remain unaffected and can be successfully modified through laser postmodification. The cells tests indicated that the micro-modifications created induced MG63 and MC3T3 osteoblast cellular orientation. The analysis of the MG63 and MC3T3 osteoblast attachment suggested regulation of cells volume migration.
Author A. Daskalova - [Institute of Electronics Bulgarian Academy of Sciences]
A. Daskalova,,
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, Barbara Ostrowska (FMSE / DMD)
Barbara Ostrowska,,
- Division of Materials Design
, A. Zhelyazkova - [Institute of Electronics Bulgarian Academy of Sciences]
A. Zhelyazkova,,
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, Wojciech Święszkowski (FMSE / DMD)
Wojciech Święszkowski,,
- Division of Materials Design
, A. Trifonov - [Sofia University St. Kliment Ohridski]
A. Trifonov,,
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, H. Declercq - [Universiteit Gent]
H. Declercq,,
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, C.S.R. Nathala - [High Q Laser GmbH]
C.S.R. Nathala,,
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, Karol Szlązak (FMSE / DMD)
Karol Szlązak,,
- Division of Materials Design
, Maciej Łojkowski (FMSE / DMD)
Maciej Łojkowski,,
- Division of Materials Design
, W. Husinsky - [Technische Universitat Wien]
W. Husinsky,,
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et al.`
Journal seriesApplied Physics A-Materials Science & Processing, ISSN 0947-8396, (A 25 pkt)
Issue year2018
Vol124
No6
Pages413-428
Publication size in sheets0.75
ASJC Classification2500 General Materials Science; 1600 General Chemistry
DOIDOI:10.1007/s00339-018-1831-y
Languageen angielski
File
Daskalova2018_Article_ImprovingOsteoblastsCellsProli.pdf 3.04 MB
Score (nominal)25
ScoreMinisterial score = 25.0, 25-04-2019, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 11-03-2019, ArticleFromJournal
Publication indicators Scopus Citations = 0; WoS Citations = 1; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 0.756; WoS Impact Factor: 2017 = 1.604 (2) - 2017=1.573 (5)
Citation count*2 (2019-08-09)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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