Three-dimensional nanofibrous polystyrene scaffolds modify macrophage phenotypes and activate macrophage angiogenic potential
Radosław Olkowski , Elżbieta Czarnowska , Michał Wojasiński , Justyna Niderla-Bielińska , Tomasz Ciach , Anna Ratajska
Abstract3D scaffolds represent an attractive substrate for studying macrophage activation and modification since they mimicextracellular matrix (ECM). However, macrophage response to such materials, particularly with respect to angiogenicpotential is still poorly recognized. Therefore, we investigated the effect of 3D nanofibrous polystyrene scaffolds (NPSs) versustissue culture polystyrene (TCPS) on THP-1-derived macrophages in various environmental conditions, for example,standard (m0), pro-inflammatory (m1), or anti-inflammatory (m2) with respect to pro-angiogenic potential. There were nodifferences in the expression of TNF-aand IL-10 mRNAs and respective proteins in cells cultured on NPSs compared withflatpolystyrene (TCPS), however, NPSs induced an increased VEGF production by macrophages cultured in m0 and m1 media.Cells cultured in m1, and m2 conditions secreted elevated amounts of TNF-aand IL-10, respectively, irrespective of substratesurface geometry. Each macrophage population contains large, medium, and small cells. Moreover, there were significantdifferences in the proportion of large to small macrophages depending on the medium composition, that is, in m0, m1, and m2media these proportions were 1:4, 1:3, and 1:10, respectively. The ultrastructure and the immunoexpression of TNF-aandIL-10 were analyzed under a confocal microscope. The results demonstrated differences in cell ultrastructure and suggestedthat the larger cells were pro-inflammatory macrophages, while the smaller cells were anti-inflammatory macrophages.In conclusion, NPSs activate macrophage pro-angiogenic potential. In addition, an increase in the proportion ofpro-inflammatory macrophages relative to anti-inflammatory ones in a given population favors this potential.
|Journal series||Cell Biology International, ISSN 1065-6995|
|Publication size in sheets||0.65|
|Keywords in English||angiogenesis, IL-10, macrophages, nanofibrous scaffold, VEGF, TNF-α|
|Score||= 70.0, 07-04-2020, ArticleFromJournal|
|Publication indicators||= 0; = 0; : 2018 = 0.564; : 2018 = 2.127 (2) - 2018=1.899 (5)|
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