Three-dimensional nanofibrous polystyrene scaffolds modify macrophage phenotypes and activate macrophage angiogenic potential

Radosław Olkowski , Elżbieta Czarnowska , Michał Wojasiński , Justyna Niderla-Bielińska , Tomasz Ciach , Anna Ratajska

Abstract

3D scaffolds represent an attractive substrate for studying macrophage activation and modification since they mimicextracellular matrix (ECM). However, macrophage response to such materials, particularly with respect to angiogenicpotential is still poorly recognized. Therefore, we investigated the effect of 3D nanofibrous polystyrene scaffolds (NPSs) versustissue culture polystyrene (TCPS) on THP-1-derived macrophages in various environmental conditions, for example,standard (m0), pro-inflammatory (m1), or anti-inflammatory (m2) with respect to pro-angiogenic potential. There were nodifferences in the expression of TNF-aand IL-10 mRNAs and respective proteins in cells cultured on NPSs compared withflatpolystyrene (TCPS), however, NPSs induced an increased VEGF production by macrophages cultured in m0 and m1 media.Cells cultured in m1, and m2 conditions secreted elevated amounts of TNF-aand IL-10, respectively, irrespective of substratesurface geometry. Each macrophage population contains large, medium, and small cells. Moreover, there were significantdifferences in the proportion of large to small macrophages depending on the medium composition, that is, in m0, m1, and m2media these proportions were 1:4, 1:3, and 1:10, respectively. The ultrastructure and the immunoexpression of TNF-aandIL-10 were analyzed under a confocal microscope. The results demonstrated differences in cell ultrastructure and suggestedthat the larger cells were pro-inflammatory macrophages, while the smaller cells were anti-inflammatory macrophages.In conclusion, NPSs activate macrophage pro-angiogenic potential. In addition, an increase in the proportion ofpro-inflammatory macrophages relative to anti-inflammatory ones in a given population favors this potential.
Author Radosław Olkowski - [Medical University of Warsaw]
Radosław Olkowski,,
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, Elżbieta Czarnowska - Instytut „Pomnik – Centrum Zdrowia Dziecka” (CZD) [Instytut Pomnik-Centrum Zdrowia Dziecka]
Elżbieta Czarnowska,,
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, Michał Wojasiński (FCPE / DBBE)
Michał Wojasiński,,
- Department of Biotechnology and Bioprocess Engineering
, Justyna Niderla-Bielińska - [Medical University of Warsaw]
Justyna Niderla-Bielińska,,
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, Tomasz Ciach (FCPE / DBBE)
Tomasz Ciach,,
- Department of Biotechnology and Bioprocess Engineering
, Anna Ratajska - [Medical University of Warsaw]
Anna Ratajska,,
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Journal seriesCell Biology International, ISSN 1065-6995, (N/A 70 pkt)
Issue year2019
Vol43
No3
Pages265-278
Publication size in sheets0.65
Keywords in Englishangiogenesis, IL-10, macrophages, nanofibrous scaffold, VEGF, TNF-α
ASJC Classification1307 Cell Biology; 2700 General Medicine
DOIDOI:10.1002/cbin.11094
URL https://onlinelibrary.wiley.com/doi/full/10.1002/cbin.11094
Languageen angielski
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Olkowski R. (i in.) - Three‐dimensional nanofibrous polystyrene....pdf of 28-03-2019
2.72 MB
Score (nominal)70
Score sourcejournalList
ScoreMinisterial score = 70.0, 20-10-2019, ArticleFromJournal
Publication indicators Scopus Citations = 0; WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 0.497; WoS Impact Factor: 2017 = 1.936 (2) - 2017=1.845 (5)
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