Cytotoxicity studies of selected cadmium-based quantum dots on 2Dvs.3D cell cultures

Ilona Grabowska-Jadach , Agnieszka Żuchowska , Marta Olesik , Marcin Drozd , Mariusz Pietrzak , Elżbieta Malinowska , Zbigniew Brzózka

Abstract

n our work, the cytotoxicity of selected, cadmium-based quantum dots with various surface architectures was studied on 3D spheroids. A specially designed microsystem as a tool for three-dimensional cell culture and nanoparticle toxicity evaluation was used for this aim. Two types of hydrophilic quantum dots with different surface charges at physiological pH were examined: CdTe-capped with ω-mercaptocarboxylic acid (COO−-terminated) and CdSeS/ZnS-glucosamine (–OH-terminated). We studied the influence of five different concentrations of nanoparticles within the range of 5 to 100 μM in order to assess dose-dependent toxic effects of the selected quantum dots on cellular spheroids as a more realistic (in vivo-like) model of human tissue. The obtained results of cytotoxicity were compared with the results for a standard, two-dimensional model – a cell monolayer. In the case of three-dimensional structures of various cell lines (normal MRC-5 and tumor A549) no significant differences in cytotoxicity caused by the tested nanoparticles have been noticed. The comparative studies revealed the enhanced biocompatibility of CdSeS/ZnS-OH quantum dots resulting from the presence of an uncharged ligand of biomimetic character on their surface. It was also found that the cytotoxicity of the same quantum dots depends on the cell culture model on which the tests were conducted. Significantly higher cytotoxicity of the tested nanomaterial was observed when experiments were carried out with the use of cell monolayers. Based on the obtained results, we claim that cytotoxicity studies of nanomaterials conducted on standard 2D cell monolayers are overestimated. In our opinion, reliable in vitro studies on the biological activity of nanoparticles require application of 3D cell cultures.
Author Ilona Grabowska-Jadach (FC / IBC / DM)
Ilona Grabowska-Jadach,,
- Department Of Microbioanalytics
, Agnieszka Żuchowska (FC / IBC / DM)
Agnieszka Żuchowska,,
- Department Of Microbioanalytics
, Marta Olesik
Marta Olesik,,
-
, Marcin Drozd (FC / IBC / DM)
Marcin Drozd,,
- Department Of Microbioanalytics
, Mariusz Pietrzak (FC / IBC / DM)
Mariusz Pietrzak,,
- Department Of Microbioanalytics
, Elżbieta Malinowska (FC / IBC / DM)
Elżbieta Malinowska,,
- Department Of Microbioanalytics
, Zbigniew Brzózka (FC / IBC / DM)
Zbigniew Brzózka,,
- Department Of Microbioanalytics
Journal seriesNew Journal of Chemistry, ISSN 1144-0546, (A 30 pkt)
Issue year2018
Vol42
No15
Pages12787-12795
Publication size in sheets0.5
Keywords in Englishquantum dots, microsystem, cytotoxicity, cell culture
DOIDOI:10.1039/C8NJ01986J
URL https://pubs.rsc.org/en/content/articlelanding/2018/nj/c8nj01986j#!divAbstract
Languageen angielski
File
wdpb_publikacje_pliki_plik_publikacja_3481_org.pdf 3.55 MB
Score (nominal)30
ScoreMinisterial score = 30.0, 06-08-2018, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 06-08-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.269 (2) - 2016=3.103 (5)
Citation count*
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back