Printed carbon based interface for protein immobilization

Grzegorz Wróblewski , Marta Janczuk-Richter , Tomasz Wołkowicz , Małgorzata Jakubowska , Joanna Niedziolka-Jonsson

Abstract

The aim of the investigations was to find a method of protein immobilisation in screen printed graphite layers. Three commonly used graphite powders were used to prepare conductive layers via screen printing. Several printing pastes with different carbon to polymer resin (polymethylmethacrylate) ratios were tested and the composition with the best electrical properties was selected for further investigations related to green fluorescent protein immobilisation. Six different procedures of protein immobilisation were examined including physical adsorption, electrochemical generation of carboxylic groups and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide activation, graphite functionalisation with succinic anhydride and graphite functionalisation with 3-(triethoxysilyl)propylsuccinic anhydride. Some of the functionalisation procedures were done before printing (functionalisation of graphite powders) and the others were done on printed and cured coatings. Printed graphite layers with immobilised green fluorescent proteins were studied with confocal fluorescent microscopy to assess the efficiency of the immobilisation procedures. The best results were observed for graphite functionalisation with succinic anhydride and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide activation of carboxylic groups.
Author Grzegorz Wróblewski (FM / IMBE)
Grzegorz Wróblewski,,
- The Institute of Metrology and Biomedical Engineering
, Marta Janczuk-Richter - Institute of Physical Chemistry Polish Academy of Sciences (IChF PAN) [Polska Akademia Nauk (PAN)]
Marta Janczuk-Richter,,
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, Tomasz Wołkowicz - National Institute of Public Health - National Institute of Hygiene (NIPH - NIH)
Tomasz Wołkowicz,,
-
, Małgorzata Jakubowska (FM / IMBE)
Małgorzata Jakubowska,,
- The Institute of Metrology and Biomedical Engineering
, Joanna Niedziolka-Jonsson - Institute of Physical Chemistry Polish Academy of Sciences (IChF PAN) [Polska Akademia Nauk (PAN)]
Joanna Niedziolka-Jonsson,,
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Journal seriesJournal of Materials Science-Materials in Electronics, ISSN 0957-4522, (N/A 70 pkt)
Issue year2019
Vol30
No13
Pages1-10
Publication size in sheets0.5
ASJC Classification2208 Electrical and Electronic Engineering; 2504 Electronic, Optical and Magnetic Materials; 3104 Condensed Matter Physics; 3107 Atomic and Molecular Physics, and Optics
DOIDOI:10.1007/s10854-019-01606-4
URL https://link.springer.com/article/10.1007%2Fs10854-019-01606-4
Languageen angielski
Score (nominal)70
Score sourcejournalList
ScoreMinisterial score = 70.0, 26-11-2019, ArticleFromJournal
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.705; WoS Impact Factor: 2018 = 2.195 (2) - 2018=1.96 (5)
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