Correlation between porous texture and cell seeding efficiency of gas foaming and microfluidic foaming scaffolds

M. Constantini , C. Colosi , P. Mozetic , Jakub Jaroszewicz , A. Tosato , A. Rainer , M. Trombetta , Wojciech Święszkowski , M. Dentini , Andrea Barbetta


In the design of scaffolds for tissue engineering applications, morphological parameters such as pore size, shape, and interconnectivity, as well as transport properties, should always be tailored in view of their clinical application. In this work, we demonstrate that a regular and ordered porous texture is fundamental to achieve an even cell distribution within the scaffold under perfusion seeding. To prove our hypothesis, two sets of alginate scaffolds were fabricated using two different technological approaches of the same method: gas-in-liquid foam templating. In the first one, foam was obtained by insufflating argon in a solution of alginate and a surfactant under stirring. In the second one, foam was generated inside a flow-focusing microfluidic device under highly controlled and reproducible conditions. As a result, in the former case the derived scaffold (GF) was characterized by polydispersed pores and interconnects, while in the latter (μFL), the porous structure was highly regular both with respect to the spatial arrangement of pores and interconnects and their monodispersity. Cell seeding within perfusion bioreactors of the two scaffolds revealed that cell population inside μFL scaffolds was quantitatively higher than in GF. Furthermore, seeding efficiency data for μFL samples were characterized by a lower standard deviation, indicating higher reproducibility among replicates. Finally, these results were validated by simulation of local flow velocity (CFD) inside the scaffolds proving that μFL was around one order of magnitude more permeable than GF.
Author M. Constantini
M. Constantini,,
, C. Colosi - [Universita degli Studi di Roma La Sapienza]
C. Colosi,,
, P. Mozetic - [Università Campus Bio-Medico di Roma]
P. Mozetic,,
, Jakub Jaroszewicz (FMSE)
Jakub Jaroszewicz,,
- Faculty of Materials Science and Engineering
, A. Tosato - [Universita degli Studi di Roma La Sapienza]
A. Tosato,,
, A. Rainer - [Università Campus Bio-Medico di Roma]
A. Rainer,,
, M. Trombetta - [Università Campus Bio-Medico di Roma]
M. Trombetta,,
- Università Campus Bio-Medico di Roma, Rome, Italy
, Wojciech Święszkowski (FMSE / DMD)
Wojciech Święszkowski,,
- Division of Materials Design
, M. Dentini - [Universita degli Studi di Roma La Sapienza]
M. Dentini,,
, Andrea Barbetta - Sapienza University of Rome [Universita degli Studi di Roma La Sapienza]
Andrea Barbetta,,
Journal seriesMaterials Science & Engineering C-Materials for Biological Applications, ISSN 0928-4931
Issue year2016
Publication size in sheets0.3
Keywords in EnglishMicrofluidic foaming; Porous scaffolds; Cell seeding efficiency; Bioreactor
ASJC Classification2210 Mechanical Engineering; 2211 Mechanics of Materials; 3104 Condensed Matter Physics; 2500 General Materials Science
Languageen angielski
Score (nominal)30
Score sourcejournalList
ScoreMinisterial score = 30.0, 17-09-2020, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 17-09-2020, ArticleFromJournal
Publication indicators Scopus Citations = 29; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.335; WoS Impact Factor: 2016 = 4.164 (2) - 2016=3.926 (5)
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