Miscanthus straw as substrate for biosuccinic acid production: Focusing on pretreatment and downstream processing

Katarzyna Dąbkowska , Merlin Alvarado-Morales , Mariusz Kuglarz , Irini Angelidaki


The main aim of this study was to optimize pretreatment strategies of Miscanthus × giganteus for biosuccinic acid production. A successful pretreatment with organosolv method (80% w/w of glycerol, 1.25% of H2SO4), prevented sugars conversion to furfurals and organic acids, and thereby resulted in high sugar recovery (glucan > 98%, xylan > 91%) and biomass delignification (60%). Pretreated biomass was subjected to hydrolysis with various cellulolytic enzyme cocktails (Viscozyme® L, Carezyme 1000L®, β-Glucanase, Cellic® CTec2, Cellic® HTec2). The most effective enzymes mixture composed of Cellic® CTec2 (10% w/w), β-Glucanase (5% w/w) and Cellic® HTec2 (1% w/w) resulted in high glucose (93.1%) and xylose (69.2%) yields after glycerol-based pretreatment. Succinic acid yield of 75–82% was obtained after hydrolysates fermentation, using Actinobacillus succinogenes 130Z. Finally a successful downstream concept for succinic acid purification was proposed. The succinic acid recovery with high purity (>98%) was developed.
Author Katarzyna Dąbkowska (FCPE / DBBE)
Katarzyna Dąbkowska,,
- Department of Biotechnology and Bioprocess Engineering
, Merlin Alvarado-Morales
Merlin Alvarado-Morales,,
, Mariusz Kuglarz
Mariusz Kuglarz,,
, Irini Angelidaki
Irini Angelidaki,,
Journal seriesBioresource Technology, ISSN 0960-8524
Issue year2019
Publication size in sheets0.5
Keywords in Englishdelignification, glycerol pretreatment, miscanthus biomass, succinic acid, succinic acid recovery
ASJC Classification2700 General Medicine; 1502 Bioengineering; 2105 Renewable Energy, Sustainability and the Environment; 2305 Environmental Engineering; 2311 Waste Management and Disposal
URL https://www.scopus.com/record/display.uri?eid=2-s2.0-85060273968&origin=resultslist&sort=plf-f&src=s&sid=28c83f72fb93b797f3882a03ede68c5c&sot=autdocs&sdt=autdocs&sl=18&s=AU-ID%2855628191300%29&relpos=0&citeCnt=0&searchTerm=
Languageen angielski
Score (nominal)140
Score sourcejournalList
ScoreMinisterial score = 140.0, 10-06-2020, ArticleFromJournal
Publication indicators WoS Citations = 5; Scopus Citations = 8; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.824; WoS Impact Factor: 2018 = 6.669 (2) - 2018=6.589 (5)
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