Miscanthus straw as substrate for biosuccinic acid production: Focusing on pretreatment and downstream processing
Katarzyna Dąbkowska , Merlin Alvarado-Morales , Mariusz Kuglarz , Irini Angelidaki
AbstractThe main aim of this study was to optimize pretreatment strategies of Miscanthus × giganteus for biosuccinic acid production. A successful pretreatment with organosolv method (80% w/w of glycerol, 1.25% of H2SO4), prevented sugars conversion to furfurals and organic acids, and thereby resulted in high sugar recovery (glucan > 98%, xylan > 91%) and biomass delignification (60%). Pretreated biomass was subjected to hydrolysis with various cellulolytic enzyme cocktails (Viscozyme® L, Carezyme 1000L®, β-Glucanase, Cellic® CTec2, Cellic® HTec2). The most effective enzymes mixture composed of Cellic® CTec2 (10% w/w), β-Glucanase (5% w/w) and Cellic® HTec2 (1% w/w) resulted in high glucose (93.1%) and xylose (69.2%) yields after glycerol-based pretreatment. Succinic acid yield of 75–82% was obtained after hydrolysates fermentation, using Actinobacillus succinogenes 130Z. Finally a successful downstream concept for succinic acid purification was proposed. The succinic acid recovery with high purity (>98%) was developed.
|Journal series||Bioresource Technology, ISSN 0960-8524|
|Publication size in sheets||0.5|
|Keywords in English||delignification, glycerol pretreatment, miscanthus biomass, succinic acid, succinic acid recovery|
|ASJC Classification||; ; ; ;|
|Score||= 140.0, 08-04-2020, ArticleFromJournal|
|Publication indicators||= 5; : 2018 = 1.824; : 2018 = 6.669 (2) - 2018=6.589 (5)|
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