Miscanthus straw as substrate for biosuccinic acid production: Focusing on pretreatment and downstream processing

Katarzyna Dąbkowska , Merlin Alvarado-Morales , Mariusz Kuglarz , Irini Angelidaki

Abstract

The main aim of this study was to optimize pretreatment strategies of Miscanthus × giganteus for biosuccinic acid production. A successful pretreatment with organosolv method (80% w/w of glycerol, 1.25% of H2SO4), prevented sugars conversion to furfurals and organic acids, and thereby resulted in high sugar recovery (glucan > 98%, xylan > 91%) and biomass delignification (60%). Pretreated biomass was subjected to hydrolysis with various cellulolytic enzyme cocktails (Viscozyme® L, Carezyme 1000L®, β-Glucanase, Cellic® CTec2, Cellic® HTec2). The most effective enzymes mixture composed of Cellic® CTec2 (10% w/w), β-Glucanase (5% w/w) and Cellic® HTec2 (1% w/w) resulted in high glucose (93.1%) and xylose (69.2%) yields after glycerol-based pretreatment. Succinic acid yield of 75–82% was obtained after hydrolysates fermentation, using Actinobacillus succinogenes 130Z. Finally a successful downstream concept for succinic acid purification was proposed. The succinic acid recovery with high purity (>98%) was developed.
Author Katarzyna Dąbkowska (FCPE / DBBE)
Katarzyna Dąbkowska,,
- Department of Biotechnology and Bioprocess Engineering
, Merlin Alvarado-Morales
Merlin Alvarado-Morales,,
-
, Mariusz Kuglarz
Mariusz Kuglarz,,
-
, Irini Angelidaki
Irini Angelidaki,,
-
Journal seriesBioresource Technology, ISSN 0960-8524, (A 45 pkt)
Issue year2019
Vol278
Pages82-91
Publication size in sheets0.5
Keywords in Englishdelignification, glycerol pretreatment, miscanthus biomass, succinic acid, succinic acid recovery
ASJC Classification2311 Waste Management and Disposal; 2105 Renewable Energy, Sustainability and the Environment; 2700 General Medicine; 2305 Environmental Engineering; 1502 Bioengineering
DOIDOI:10.1016/j.biortech.2019.01.051
URL https://www.scopus.com/record/display.uri?eid=2-s2.0-85060273968&origin=resultslist&sort=plf-f&src=s&sid=28c83f72fb93b797f3882a03ede68c5c&sot=autdocs&sdt=autdocs&sl=18&s=AU-ID%2855628191300%29&relpos=0&citeCnt=0&searchTerm=
Languageen angielski
Score (nominal)45
ScoreMinisterial score = 45.0, 08-05-2019, ArticleFromJournal
Ministerial score (2013-2016) = 45.0, 11-03-2019, ArticleFromJournal
Publication indicators WoS Citations = 1; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.91; WoS Impact Factor: 2017 = 5.807 (2) - 2017=5.978 (5)
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