Spatially multiplexed interferometric microscopy: principles and applications to biomedical imaging
Authors:
- José Ángel Picazo-Bueno,
- Maciej Trusiak,
- Javier García,
- Vicente Micó
Abstract
Digital holographic microscopy (DHM) applied to quantitative phase imaging (QPI) has been successfully demonstrated as a powerful label-free method to analyse the optical properties of cells. Spatially multiplexed interferometric microscopy (SMIM) is a DHM technique that implements a common-path interferometric layout in the embodiment of a standard microscope to achieve QPI. More concretely, SMIM introduces three minimal modifications: (a) replaces the broadband illumination of the microscope by a coherent or partially coherent light source, (b) divides the input plane into two or three regions for transmission in parallel of both imaging and reference beams, and (c) includes a one-dimensional (1D) diffraction grating or a beam splitter cube for holographic recording. Hence, SMIM is a cost-effective, extremely simple, and highly stable manner of converting a standard bright field microscope into a holographic one. The goal of this contribution is to present the SMIM approaches implemented using a 1D diffraction grating, and highlight vast range of capabilities for biomedical imaging including super-resolved, reflective, transflective, noise-reduced and single-shot slightly off-axis amplitude and phase imaging.
- Record ID
- WUTdfe80efd79a6487c8df16e6054cf9884
- Author
- Journal series
- Journal of Physics: Photonics, ISSN , e-ISSN 2515-7647
- Issue year
- 2021
- Vol
- 3
- No
- 3
- Pages
- 1-16
- Article number
- 034005
- Keywords in English
- digital holographic microscopy, quantitative phase imaging, medical and biological imaging, interference microscopy, phase retrieval, digital image processing
- DOI
- DOI:10.1088/2515-7647/abfc59 Opening in a new tab
- URL
- https://iopscience.iop.org/article/10.1088/2515-7647/abfc59 Opening in a new tab
- Language
- eng (en) English
- License
- File
-
- File: 1
- Spatially multiplexed interferometric microscopy: principles and applications to biomedical imaging, File WUTd7cdbdfb22f949baaffc9c3c7978c613.pdf / 1 MB
- WUTd7cdbdfb22f949baaffc9c3c7978c613.pdf
- publication date: 08-04-2022
- Spatially multiplexed interferometric microscopy: principles and applications to biomedical imaging, File WUTd7cdbdfb22f949baaffc9c3c7978c613.pdf / 1 MB
-
- Score (nominal)
- 20
- Score source
- journalList
- Score
- = 20.0, 26-04-2022, ArticleFromJournal
- Publication indicators
- = 0; = 2
- Citation count
- 2
- Uniform Resource Identifier
- https://repo.pw.edu.pl/info/article/WUTdfe80efd79a6487c8df16e6054cf9884/
- URN
urn:pw-repo:WUTdfe80efd79a6487c8df16e6054cf9884
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or PerishOpening in a new tab system.