Alignment and bioactive molecule enrichment of bio-composite scaffolds towards peripheral nerve tissue engineering

Ewa Kijeńska-Gawrońska , Tomasz Bolek , Monika Bil , Wojciech Święszkowski

Abstract

Providing topographical cues along with chemical and biological factors is essential for biomimetic scaffolds applied in nerve tissue engineering. The aim of the presented work was to study the synergistic effect of the surface's topography and biological stimulation using nerve growth factor (NGF) on the behavior of PC12 cells. Three types of scaffolds based on poly(l-lactide-co-caprolactone), collagen type I and collagen type III with aligned orientation of the fibers were fabricated by either blend or coaxial electrospinning. The chemical, physical and mechanical properties of the scaffolds were further evaluated and, at the same time, the influence of the NGF and BSA incorporation method on the release profile was investigated. Moreover, the NGF dependent PC12 cell response was analyzed. It was found that encapsulation of the growth factor within the core of core-shell nanofibers enables preservation of the growth factor bio-activity. On the other hand, nanofibers produced by co-axial electrospinning allowed for control and stable release, which can be described by the Higuchi and Korsmeyer-Peppas models. This results in PC12 interaction with the material, and it was observed that the cells differentiated more efficiently, expressing bi- and tri-polar elongations, retaining their typical phenotype, on core-shell scaffolds compared to the other ones utilized within this work. The presented studies indicate that poly(l-lactide-co-caprolactone), collagen type I and collagen type III scaffolds with aligned core-shell fibers are promising materials and can be applied as substrates for peripheral nerve regeneration. © 2019 The Royal Society of Chemistry.
Author Ewa Kijeńska-Gawrońska (FMSE / DMD)
Ewa Kijeńska-Gawrońska,,
- Division of Materials Design
, Tomasz Bolek (FMSE / DMD)
Tomasz Bolek,,
- Division of Materials Design
, Monika Bil (FMSE / DMD)
Monika Bil,,
- Division of Materials Design
, Wojciech Święszkowski (FMSE / DMD)
Wojciech Święszkowski,,
- Division of Materials Design
Journal seriesJournal of Materials Chemistry B: materials for biology and medicine, ISSN 2050-750X, e-ISSN 2050-7518, (N/A 140 pkt)
Issue year2019
Vol7
No29
Pages4509-4519
Publication size in sheets0.5
DOIDOI:10.1039/C9TB00367C
URL https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069775891&doi=10.1039%2fc9tb00367c&partnerID=40&md5=ba74de3139692b7e81edbc7b5e2af7c3
Languageen angielski
File
c9tb00367c.pdf 5.37 MB
Score (nominal)140
Score sourcejournalList
ScoreMinisterial score = 140.0, 10-01-2020, ArticleFromJournal
Publication indicators WoS Citations = 0; WoS Impact Factor: 2018 = 5.047 (2) - 2018=5.003 (5)
Citation count*
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back
Confirmation
Are you sure?