Single-shot slightly off-axis digital holographic microscopy with add-on module based on beamsplitter cube

José Ángel Picazo-Bueno , Maciej Trusiak , Micó Vicente

Abstract

3. Slightly off-axis digital holographic microscopy (SO-DHM) has recently emerged as a novel experimental arrangement for quantitative phase imaging (QPI). It offers improved capabilities in conventional on-axis and off-axis interferometric configurations. In this contribution, we report on a single-shot SO-DHM approach based on an add-on module adapted to the exit port of a regular microscope. The module employs a beamsplitter (BS) cube interferometer and includes, in addition, a Stokes lens (SL) for astigmatism compensation. Each recorded frame contains two fields of view (FOVs) of the sample, where each FOV is a hologram which is phase shifted by π rads with respect to the other. These two simultaneously recorded holograms are numerically processed, in order to retrieve complex amplitude distribution with enhanced quality. The tradeoff is done in the FOV which becomes penalized as a consequence of the simultaneous recording of the two holograms in a single snapshot. Experimental validation is presented for a wide variety of samples using a regular Olympus BX-60 upright microscope. The proposed approach provides an optimized use of the imaging system, in terms of the space-bandwidth product, in comparison with off-axis configuration; allows the analysis of fast-dynamic events, owing to its single-shot capability when compared with on-axis arrangement; and becomes easily implementable in conventional white-light microscopes for upgrading them into holographic microscopes for QPI.
Author José Ángel Picazo-Bueno - University of Valencia
José Ángel Picazo-Bueno,,
-
, Maciej Trusiak (FM / IMPh)
Maciej Trusiak,,
- The Institute of Micromechanics and Photonics
, Micó Vicente - University of Valencia
Micó Vicente,,
-
Journal seriesOptics Express, ISSN 1094-4087, (A 40 pkt)
Issue year2019
Vol27
No4
Pages5655-1 - 5655-15
Publication size in sheets0.3
Keywords in Englishimage quality, live cell imaging, optical components, optical elements, phase imaging, phase retrieval
ASJC Classification3107 Atomic and Molecular Physics, and Optics
DOIDOI:10.1364/OE.27.005655
URL https://www.osapublishing.org/oe/abstract.cfm?uri=oe-27-4-5655&origin=search
projectNumerical advanced phase and amplitude demodulation for optical interference and tomography. Project leader: Trusiak Maciej, , Phone: +48 22234-8637, start date 19-01-2018, planned end date 18-01-2021, 505/00792, Implemented
WM Projects financed by NSC [Projekty finansowane przez NCN]
Languageen angielski
Score (nominal)40
ScoreMinisterial score = 40.0, 15-05-2019, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.567; WoS Impact Factor: 2017 = 3.356 (2) - 2017=3.372 (5)
Citation count*1 (2019-07-11)
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