Hybrid de novo whole-genome assembly and annotation of the model tapeworm Hymenolepis diminuta
Robert Marek Nowak , Jakub Jastrzębski , Wiktor Kuśmirek , Rusłan Sałamatin , Małgorzata Rydzanicz , Agnieszka Sobczyk-Kopcioł , Anna Sulima-Celińska , Łukasz Paukszto , Karol G. Makowczenko , Rafał Płoski , Vasyl V. Tkach , Katarzyna Basałaj , Daniel Młocicki
AbstractDespite the use of Hymenolepis diminuta as a model organism in experimental parasitology, a full genome description has not yet been published. Here we present a hybrid de novo genome assembly based on complementary sequencing technologies and methods. The combination of Illumina paired-end, Illumina mate-pair and Oxford Nanopore Technology reads greatly improved the assembly of the H. diminuta genome. Our results indicate that the hybrid sequencing approach is the method of choice for obtaining high-quality data. The final genome assembly is 177 Mbp with contig N50 size of 75 kbp and a scaffold N50 size of 2.3 Mbp. We obtained one of the most complete cestode genome assemblies and annotated 15,169 potential protein-coding genes. The obtained data may help explain cestode gene function and better clarify the evolution of its gene families, and thus the adaptive features evolved during millennia of co-evolution with their hosts.
|Journal series||Scientific data, ISSN 2052-4463, (N/A 140 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Mitochondrial genome, Eukaryote, DNA sequencing|
|ASJC Classification||; ; ; ; ;|
|Score||= 140.0, 01-01-2020, ArticleFromJournal|
|Publication indicators||= 0; : 2018 = 2.629; : 2017 = 5.305 (2) - 2017=5.862 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.