DNA changes in Pseudomonas putida induced by aluminum oxide nanoparticles using RAPD analysis

Monika Załęska-Radziwiłł , Nina Doskocz

Abstract

This research has been conducted to determine the genotoxicity of nano-Al2O3 towards Pseudomonas putida bacteria using RAPD-PCR method. The results were compared with the impact of the macro form of the compound on DNA. On the basis of RAPD profiles using the primer OPA2, the research demonstrated a mutagenic action of the tested nanocompound. The obtained profiles of RAPD bands are different from the negative control by over 30–9.1%. For the products of PCR carried out using the primer OPA9, genetic similarity indexes relative to the negative control for nano-Al2O3 samples were 80% in all tested concentrations. Regarding the remaining primers, RAPD bands profiles for the samples with the nanocompound and the negative control varied only to a small extent. This study showed a decrease in the genetic stability of DNA (GTS, %) after treatment of the bacteria nano-Al2O3 as compared with the negative control. GTS value for the nano-Al2O3 was 65% (1,000 mg/L). In the remaining concentrations of nano-Al2O3, GTS remained in the range of 75–80%. The results showed that the nano-Al2O3 can induce modifications of the genetic material to a greater extent than the same compounds in the macro form.
Author Monika Załęska-Radziwiłł ZB
Monika Załęska-Radziwiłł,,
- Department of Biology
, Nina Doskocz ZB
Nina Doskocz,,
- Department of Biology
Journal seriesDesalination and Water Treatment, ISSN 1944-3994
Issue year2016
Vol57
No3
Pages1573-1581
Publication size in sheets0.5
Keywords in EnglishAluminum oxide nanoparticles, RAPD-PCR, Genotoxicity
DOIDOI:10.1080/19443994.2014.996015
Languageen angielski
Score (nominal)20
ScoreMinisterial score = 20.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 20.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 1.631 (2) - 2016=1.643 (5)
Citation count*6 (2018-07-20)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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