Enzymatic activity of commercial cellulolytic enzyme preparations towards various biomass pre-treated in high-temperature/high-pressure

Katarzyna Dąbkowska , Kamil Kopeć , Maciej Pilarek

Abstract

Enzymatic hydrolysis is the essential step in the production of II generation biofuels made from lignocellulosic biomass, i.e. agricultural and forestry wastes. The efficient enzyme-catalyzed degradation of cellulose and hemicellulose to monosaccharides requires the synergistic action of the independent types of enzymes. The basic aim of the study was to experimentally determine the activity of two commercially offered cellulolytic enzyme preparations: Cellic®CTec2 and the mixture of Celluclast®1.5L and Novozyme 188 in the hydrolysis of lignocellulosic biomass: energetic willow and rye straw, as well as cellulose paper used as a feedstock. Before hydrolysis, the biomass was subjected to alkaline pre-treatment at high-temperature (121°C) and high-pressure (0.1 MPa) conditions. Based on the results, the influence of the type and concentration of enzymes on the effectiveness of hydrolysis was evaluated and finally the enzyme activities were determined for each of tested cellulolytic preparations.
Author Katarzyna Dąbkowska (FCPE / DBBE)
Katarzyna Dąbkowska,,
- Department of Biotechnology and Bioprocess Engineering
, Kamil Kopeć (FCPE / DBBE)
Kamil Kopeć,,
- Department of Biotechnology and Bioprocess Engineering
, Maciej Pilarek (FCPE / DBBE)
Maciej Pilarek,,
- Department of Biotechnology and Bioprocess Engineering
Pages295-302
Publication size in sheets0.5
Book The 22nd Polish Conference of Chemical and Process Engineering: Proceedings, 2016, Łódź, Faculty of Process and Environmental Engineering, ISBN 978-83-61997-75-7, 1584 p.
Proceedings OKIChiP 2016.pdf / No licence information (file archived - login or check accessibility on faculty)
Keywords in Englishcellulolytic enzymes, lignocellulose, pre-treatment, hydrolysis
Languageen angielski
Score (nominal)0
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