“Novel designs and technologies for cell engineering”
K. Ziółkowska , Michał Chudy , Artur Dybko , Zbigniew Brzózka
AbstractMicrofluidic devices, such as lab-on-a-chip systems, are highly advantageous for cell engineering and cell based assays. It is a particularly useful approach for development of the in vitro cellular systems mimicking the in vivo environment. In this paper, a novel lab-on-a-chip device for three-dimensional human cell culture and anticancer drug testing is presented. Cells were cultured as Multicellular Tumor Spheroids (MCTS) – the best cancer tumor model developed so far. Different designs have been tested and novel technique of microfabrication in poly(dimethylsiloxane) was developed. MCTS were cultured in a system of polymeric microwells, with the network of microfluidic channels for culture medium flow. Design included optimal shear stress and proper nutrients supply for cultured cells. Final design provided MCTS culture for four weeks with the homeostasis-like state achievement, which is characteristic for the in vivo situation.
|Journal series||Challenges of Modern Technology, ISSN 2082-2863 [2353-4419]|
|Citation count*||2 (2015-08-13)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.