Adhesion of MRC-5 and A549 cells on poly(dimethylosiloxane) surface modified by proteins

Agnieszka Żuchowska , Piotr Kwiatkowski , Elżbieta Jastrzębska , Michał Chudy , Artur Dybko , Zbigniew Brzózka

Abstract

PDMS is a very popular material used for fabrication of Lab-on-a-Chip systems for biological applications. Although PDMS has numerous advantages, it is a highly hydrophobic material, which inhibits adhesion and proliferation of the cells. PDMS surface modifications are used to enrich growth of the cells. However, due to the fact that each cell type has specific adhesion, it is necessary to optimize the parameters of these modifications. In this paper, we present an investigation of normal (MRC-5) and carcinoma (A549) human lung cell adhesion and proliferation on modified PDMS surfaces. We have chosen these cell types because often they are used as models for basic cancer research. To the best of our knowledge, this is the first presentation of this type of investigation. The combination of a gas-phase processing (oxygen plasma or ultraviolet irradiation) and wet chemical methods based on proteins' adsorption was used in our experiments. Different proteins such as poly-l-lysine, fibronectin, laminin, gelatin, and collagen were incubated with the activated PDMS samples. To compare with other works, here, we also examined how ratio of prepolymer to curing agent (5:1, 10:1, and 20:1) influences PDMS hydrophilicity during further modifications. The highest adhesion of the tested cells was observed for the usage of collagen, regardless of PDMS ratio. However, the MRC-5 cell line demonstrated better adhesion than A549 cells. This is probably due to the difference in their morphology and type (normal/cancer).
Author Agnieszka Żuchowska ZMB
Agnieszka Żuchowska,,
- Department Of Microbioanalytics
, Piotr Kwiatkowski ZMB
Piotr Kwiatkowski,,
- Department Of Microbioanalytics
, Elżbieta Jastrzębska ZMB
Elżbieta Jastrzębska,,
- Department Of Microbioanalytics
, Michał Chudy ZMB
Michał Chudy,,
- Department Of Microbioanalytics
, Artur Dybko ZMB
Artur Dybko,,
- Department Of Microbioanalytics
, Zbigniew Brzózka ZMB
Zbigniew Brzózka,,
- Department Of Microbioanalytics
Journal seriesElectrophoresis, ISSN 0173-0835
Issue year2016
Vol37
No3
Pages536-544
Publication size in sheets0.5
Keywords in EnglishA549 and MRC-5 cells;Cell adhesion;Poly(dimethylsiloxane);Surface modification
DOIDOI:10.1002/elps.201500250
URL http://onlinelibrary.wiley.com/doi/10.1002/elps.201500250/abstract
Languageen angielski
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Zuchowska_et_al-2016-ELECTROPHORESIS.pdf (file archived - login or check accessibility on faculty) Zuchowska_et_al-2016-ELECTROPHORESIS.pdf 890.03 KB
Score (nominal)30
ScoreMinisterial score = 25.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.744 (2) - 2016=2.487 (5)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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