Comparison of elicitor-based effects on metabolic responses of Taxus × media hairy roots in perfluorodecalin-supported two-phase culture system

Katarzyna Sykłowska-Baranek , Wojciech Rymaszewski , Małgorzata Gaweł , P. Rokicki , Maciej Pilarek , Marta Grech-Baran , Jacek Hennig , Agnieszka Pietrosiuk

Abstract

Two lines of Taxus × media hairy roots were subjected to single or twice-repeated supplementation with methyl jasmonate, sodium nitroprusside, l-phenylalanine, and sucrose feeding. One line harboured transgene of taxadiene synthase (ATMA), while the second (KT) did not. Both hairy root lines were cultured in two-phase culture systems containing perfluorodecalin (PFD) in aerated or degassed form. The relationship between TXS (taxadiene synthase), BAPT (baccatin III: 3-amino, 3-phenylpropanoyltransferase), and DBTNBT (3′-N-debenzoyl-2-deoxytaxol-N-benzoyltransferase) genes and taxane production was analysed. The ATMA and KT lines differed in their potential for taxane accumulation, secretion, and taxane profile. In ATMA biomass, both paclitaxel and baccatin III were detected, while in KT roots only paclitaxel. The most suitable conditions for taxane production for ATMA roots were found in single-elicited supported with PFD-degassed cultures (2 473.29 ± 263.85 µg/g DW), whereas in KT roots in single-elicited cultures with PFD-aerated (470.08 ± 25.15 µg/g DW). The extracellular levels of paclitaxel never exceeded 10% for ATMA roots, while for KT increased up to 76%. The gene expression profile was determined in single-elicited cultures supported with PFD-degassed, where in ATMA roots, the highest taxane yield was obtained, while in KT the lowest one. The gene expression pattern in both investigated root lines differed substantially what resulted in taxane yield characterized particular lines. The highest co-expression of TXS, BAPT and DBTNBT genes noted for ATMA roots harvested 48 h after elicitation corresponded with their higher ability for taxane production in comparison with the effects observed for KT roots.
Author Katarzyna Sykłowska-Baranek - Medical University of Warsaw (WUM) [Medical University of Warsaw]
Katarzyna Sykłowska-Baranek,,
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, Wojciech Rymaszewski - [Institute of Biochemistry and Biophysics of the Polish Academy of Sciences]
Wojciech Rymaszewski,,
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, Małgorzata Gaweł - [Medical University of Warsaw]
Małgorzata Gaweł,,
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, P. Rokicki - [Medical University of Warsaw]
P. Rokicki,,
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, Maciej Pilarek (FCPE / DBBE)
Maciej Pilarek,,
- Department of Biotechnology and Bioprocess Engineering
, Marta Grech-Baran - [Medical University of Warsaw]
Marta Grech-Baran,,
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, Jacek Hennig - [Institute of Biochemistry and Biophysics of the Polish Academy of Sciences]
Jacek Hennig,,
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, Agnieszka Pietrosiuk - Medical University of Warsaw (WUM) [Medical University of Warsaw]
Agnieszka Pietrosiuk,,
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Journal seriesPlant Cell Reports, ISSN 0721-7714, (A 35 pkt)
Issue year2019
Vol38
No1
Pages85-99
Publication size in sheets0.7
Keywords in EnglishTaxus, hairy roots, elicitation, perfluorodecalin, gene expression
ASJC Classification1110 Plant Science; 1102 Agronomy and Crop Science; 2700 General Medicine
DOIDOI:10.1007/s00299-018-2351-0
URL https://link.springer.com/content/pdf/10.1007%2Fs00299-018-2351-0.pdf
Languageen angielski
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Sykłowska-Baranek K. (i in.) - Comparison of elicitor-based...pdf 1.2 MB
Score (nominal)35
ScoreMinisterial score = 35.0, 23-07-2019, ArticleFromJournal
Publication indicators WoS Citations = 1; Scopus Citations = 1; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.05; WoS Impact Factor: 2017 = 2.989 (2) - 2017=2.992 (5)
Citation count*1 (2019-08-03)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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