Formation of calcium phosphate coatings within polycaprolactone scaffolds by simple, alkaline phosphatase based method
Jakub Jaroszewicz , Joanna Idaszek , Emilia Choińska , Karol Szlązak , Anna Hyc , Anna Osiecka-Iwan , Wojciech Święszkowski , Stanislaw Moskalewski
AbstractThe paper presents a novel approach to the production of calcium phosphate coatings of scaffolds. Mineral deposits were formed during incubation of polycaprolactone (PCL) scaffolds with bovine intestinal alkaline phosphatase in sodium glycerophosphate and calcium chloride medium. To modify hydrophobic surface of scaffolds and intensify attachment of coating, scaffolds were incubated at 50 °C (thermal activation, TA) or at 37 °C after short exposition to lipase (lipase activation, LA). Micro-computed tomographyobservations demonstrated that both methods resulted in deposition of mineral on the surface of external and internal walls of the scaffolds. Precipitate formed after thermal and lipase activation contained particles with average size of 200–400 nm, and the shape of donuts. In thermal activated PCL coatings X-ray diffraction disclosed peaks typical for hydroxyapatite (HAp), while after lipase activation these peaks could be precisely defined only if left for 6 days in the incubation medium. The Fourier-transform infrared spectroscopysuggested crystalline structure of HAp both after thermal and lipase activation. The adherence of bone marrow mesenchymal stem cells was initially higher on coated than pristine PCL, but during 7 days of culture the cell number increased and was similar on all tested samples. Alkaline phosphatase activity, considered as a sign of osteogenic differentiation, measured on PCL samples after 7 days was 2–3 times lower on pristine PCL than on the coated samples, but after 2 weeks increased significantly and reached similar value as on the calcium phosphate substrates.
|Journal series||Materials Science & Engineering C - Materials for Biological Applications, ISSN 0928-4931, (N/A 140 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||3D scaffold; Alkaline phosphatase; Bioactive coating; Calcium phosphate; Hydroxyapatite|
|ASJC Classification||; ; ;|
|Score||= 140.0, 10-01-2020, ArticleFromJournal|
|Publication indicators||= 2; = 1; = 3.0; : 2017 = 1.384; : 2018 = 4.959 (2) - 2018=4.708 (5)|
|Citation count*||3 (2020-01-18)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.