Speciation and identification of tellurium-containing metabolites in garlic, Allium sativum

Y. Anan , M Yoshida , S. Hasegawa , R. Katai , M. Tokumoto , L. Ouerdane , Ryszard Łobiński , Y. Ogra

Abstract

Tellurium (Te) is a widely used metalloid in industry because of its unique chemical and physical properties. However, information about the biological and toxicological activities of Te in plants and animals is limited. Although Te is expected to be metabolized in organisms via the same pathway as sulfur and selenium (Se), no precise metabolic pathways are known in organisms, particularly in plants. To reveal the metabolic pathway of Te in plants, garlic, a well-known Se accumulator, was chosen as the model plant. Garlic was hydroponically cultivated and exposed to sodium tellurate, and Te-containing metabolites in the water extract of garlic leaves were identified using HPLC coupled with inductively coupled plasma mass spectrometry (ICP-MS) or electrospray tandem mass spectrometry (ESI-MS-MS). At least three Te-containing metabolites were detected using HPLC-ICP-MS, and two of them were subjected to HPLC-ESI-MS-MS for identification. The MS spectra obtained by ESI-MS-MS indicated that the metabolite was Te-methyltellurocysteine oxide (MeTeCysO). Then, MeTeCysO was chemically synthesized and its chromatographic behavior matched with that of the Te-containing metabolite in garlic. The other was assigned as cysteine S-methyltellurosulfide. These results suggest that garlic can assimilate tellurate, an inorganic Te compound, and tellurate is transformed into a Te-containing amino acid, the so-called telluroamino acid. This is the first report addressing that telluroamino acid is de novo synthesized in a higher plant.
Author Y. Anan - [Showa Pharmaceutical University]
Y. Anan,,
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, M Yoshida - [Showa Pharmaceutical University]
M Yoshida,,
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, S. Hasegawa - [Showa Pharmaceutical University]
S. Hasegawa,,
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, R. Katai - [Showa Pharmaceutical University]
R. Katai,,
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, M. Tokumoto - [Showa Pharmaceutical University]
M. Tokumoto,,
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, L. Ouerdane - [Universite de Pau et des Pays de L'Adour]
L. Ouerdane,,
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, Ryszard Łobiński (FC / CAC)
Ryszard Łobiński,,
- Chair Of Analytical Chemistry
, Y. Ogra - [Showa Pharmaceutical University]
Y. Ogra,,
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Journal seriesMetallomics, ISSN 1756-5901
Issue year2013
Vol5
No9
Pages1215-1224
Publication size in sheets0.5
ASJC Classification2506 Metals and Alloys; 1303 Biochemistry; 2502 Biomaterials; 1304 Biophysics; 1601 Chemistry (miscellaneous)
DOIDOI:10.1039/c3mt00108c
URL http://pubs.rsc.org/en/Content/ArticleLanding/2013/MT/c3mt00108c#!divAbstract
Languageen angielski
File
wdpb_publikacje_pliki_plik_publikacja_1967_org.pdf 1.62 MB
Score (nominal)35
Score sourcejournalList
ScoreMinisterial score = 35.0, 01-02-2020, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 01-02-2020, ArticleFromJournal
Publication indicators Scopus Citations = 15; WoS Citations = 14; Scopus SNIP (Source Normalised Impact per Paper): 2014 = 1.09; WoS Impact Factor: 2013 = 3.978 (2) - 2013=4.01 (5)
Citation count*4 (2015-02-27)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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