New antifungal 4-chloro-3-nitrophenyldifluoroiodomethyl sulfone reduces the Candida albicans pathogenicity in the Galleria mellonella model organism
Authors:
- Monika Staniszewska,
- Małgorzata Gizińska,
- Michalina Kazek,
- Roberto de Jesús González-Hernández,
- Zbigniew Ochal,
- Hector M. Mora-Montes
Abstract
Candida albicans represents an interesting microorganism to study complex host-pathogen interactions and for the development of effective antifungals. Our goal was to assess the efficacy of 4-chloro-3-nitrophenyldifluoroiodomethyl sulfone (named Sulfone) against the C. albicans infections in the Galleria mellonella host model. We assessed invasiveness of CAI4 parental strain and mutants: kex2Δ/KEX2 and kex2Δ/kex2Δ in G. mellonella treated with Sulfone. We determined that KEX2 expression was altered following Sulfone treatment in G. mellonella-C. albicans infection model. Infection with kex2Δ/kex2Δ induced decreased inflammation and minimal fault in fitness of larvae vs CAI4. Fifty percent of larvae died within 4–5 days (P value< 0.0001) when infected with CAI4 and kex2Δ/KEX2 at 109 CFU/mL; survival reached 100% in those injected with kex2Δ/kex2Δ. Larvae treated with Sulfone at 0.01 mg/kg 30 min before infection with all C. albicans tested survived infection at 90–100% vs C. albicans infected-PBS-treated larvae. Hypersensitive to Sulfone, kex2Δ/kex2Δ reduced virulence in survival. KEX2 was down-regulated when larvae were treated with Sulfone: 30 min before and 2 h post-SC5314-wild-type infection respectively. kex2Δ/kex2Δ was able to infect larvae, but failed to kill host when treated with Sulfone. Sulfone can be used to prevent or treat candidiasis. G. mellonella facilitates studding of host-pathogen interactions, i.e., testing host vs panel of C. albicans mutants when antifungal is dosed.
- Record ID
- WUTd319485e512047c1991b583aa28177bc
- Author
- Journal series
- Brazilian Journal of Microbiology, ISSN 1517-8382, e-ISSN 1678-4405
- Issue year
- 2020
- Vol
- 51
- Pages
- 5-14
- Keywords in English
- C.albicans, Virulence, Candidiasis, G.mellonella, Gene expression, Antifungal agent
- ASJC Classification
- DOI
- DOI:10.1007/s42770-019-00140-z Opening in a new tab
- URL
- https://link.springer.com/article/10.1007/s42770-019-00140-z Opening in a new tab
- Language
- (en) English
- License
- File
-
- File: 1
- New antifungal 4-chloro-3-nitrophenyldifluoroiodomethyl sulfone reduces the Candida albicans pathogenicity in the Galleria mellonella model organism, File wdpb_publikacje_pliki_plik_publikacja_3792_org.pdf / 1 MB
- wdpb_publikacje_pliki_plik_publikacja_3792_org.pdf
- publication date: 03-01-2020
- New antifungal 4-chloro-3-nitrophenyldifluoroiodomethyl sulfone reduces the Candida albicans pathogenicity in the Galleria mellonella model organism, File wdpb_publikacje_pliki_plik_publikacja_3792_org.pdf / 1 MB
-
- Score (nominal)
- 70
- Score source
- journalList
- Score
- = 70.0, 30-04-2022, ArticleFromJournal
- Publication indicators
- = 0; = 4; : 2018 = 1.302; : 2020 (2 years) = 2.476 - 2020 (5 years) =3.391
- Uniform Resource Identifier
- https://repo.pw.edu.pl/info/article/WUTd319485e512047c1991b583aa28177bc/
- URN
urn:pw-repo:WUTd319485e512047c1991b583aa28177bc
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or PerishOpening in a new tab system.