Modelling of a hybrid culture system with a stationary layer of liquid perfluorochemical applied as oxygen carrier

Maciej Pilarek , Katarzyna Dąbkowska


A mathematical model of a hybrid culture system supported with a stationary layer of liquid perfluorochemical (PFC) as a source of O2 for cells which grow in the aqueous phase of culture medium has been developed and discussed. The two-substrate Monod kinetics without inhibition effects, i.e. the Tsao-Hanson equation, has been assumed to characterise the biomass growth. The Damköhler number which relates the growth rate to the mass transfer effects has been used to appraise the regime (i.e. diffusion-limited or kinetics) of the whole process. The proposed model predicted accurately previously published data on the submerged batch cultures of Nicotiana tabacum BY-2 heterotrophic cells performed in a culture system supported with a stationary layer of hydrophobic perfluorodecalin as a liquid O2 carrier. Estimated values of the parameters of the model showed that the process proceeded in the kinetics regime and the growth kinetics, not the effects of the mass transfer between aqueous phase and liquid PFC, had essential influence on the growth of biomass.
Author Maciej Pilarek ZBIB
Maciej Pilarek,,
- Department of Biotechnology and Bioprocess Engineering
, Katarzyna Dąbkowska ZBIB
Katarzyna Dąbkowska,,
- Department of Biotechnology and Bioprocess Engineering
Journal seriesChemical and Process Engineering , ISSN 0208-6425
Issue year2016
Publication size in sheets0.5
Keywords in Englishliquid perfluorochemical (fluorocarbon), liquid gas carrier, mathematical modelling, cell/tissue culture, hybrid culture system
Languageen angielski
Pilarek M., Dąbkowska K. - Modelling of a....pdf 321.57 KB
Score (nominal)15
ScoreMinisterial score = 15.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 15.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 0.971 (2) - 2016=0.925 (5)
Citation count*0 (2018-06-17)
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