The toxicity in vitro of titanium dioxide nanoparticles modified with noble metals on mammalian cells

M. Rosłon , Agnieszka Jastrzębska , Karolina Sitarz , Iza Książek , Mirosława Koronkiewicz , E. Anuszewska , Małgorzata Jaworska , J. Dudkiewicz-Wilczyńska , Wanda Ziemkowska , Dariusz Basiak , Anita Rozmysłowska-Wojciechowska , Andrzej Olszyna


The aim of the presented work was to evaluate the effect of modification of tita-nium dioxide nanoparticles with noble metals and their oxides on selected mam-malian cells. The in vitro cytotoxicity studies of titanium dioxide (TiO2) nanoparticles modified with Au, Ag, Pd, Ag2O, and PdO with reference to the unmodified TiO2 nanoparticles were presented. The evaluation of cytotoxic activity of the tested nanocomposite particles was carried out using three cell lines: Caco‐2 olorectal epithelial adenocarcinoma cells, BJ normal human skin fibroblasts, and L929 mouse fibroblasts. The in vitro studies included determination of cell viability after 24 and 48 hours of exposure to the nanocomposite particles, using the MTT assay, as well as flow cytometry with Annexin V‐FITC staining. Our results indicate that irrespectively of the kind of cell line and assay used, nanoparticles of unmodified titanium dioxide as well as those with addition of gold and palladium have a slight impact on cell viability at the investigated concentration range (10‐200 μg/mL). Nanoparticles with addition of silver (Ag and Ag2O), were found to have significantly higher toxic effect, the level of wchich varied depending on the cell line and assay used
Author M. Rosłon - [National Medicines Institute, Warsaw]
M. Rosłon,,
, Agnieszka Jastrzębska (FMSE / DSMP)
Agnieszka Jastrzębska,,
- Division of Ceramic Materials and Polymers
, Karolina Sitarz - [National Medicines Institute, Warsaw]
Karolina Sitarz,,
, Iza Książek - [National Medicines Institute, Warsaw]
Iza Książek,,
, Mirosława Koronkiewicz - [National Medicines Institute, Warsaw]
Mirosława Koronkiewicz,,
, E. Anuszewska - [National Medicines Institute, Warsaw]
E. Anuszewska,,
, Małgorzata Jaworska - [National Medicines Institute, Warsaw]
Małgorzata Jaworska,,
, J. Dudkiewicz-Wilczyńska - [National Medicines Institute, Warsaw]
J. Dudkiewicz-Wilczyńska,,
, Wanda Ziemkowska (FC / DCOC)
Wanda Ziemkowska,,
- Department Of Catalysis And Organometallic Chemistry
, Dariusz Basiak (FC / DCOC)
Dariusz Basiak,,
- Department Of Catalysis And Organometallic Chemistry
et al.`
Journal seriesInternational Journal of Applied Ceramic Technology, ISSN 1546-542X
Issue year2019
Publication size in sheets0.6
Keywords in Polishbioaktywność, układy ceramika-metal, dwutlenek tytanu, toksyczność in vitro
Keywords in Englishbioactivity, ceramic-metal systems, titanium dioxide, toxicity in vitro
ASJC Classification2505 Materials Chemistry; 1406 Marketing; 3104 Condensed Matter Physics; 2503 Ceramics and Composites
Languageen angielski
The toxicity in vitro of titanium dioxide nanoparticles modified with noble metals on mammalian cells.pdf 2.28 MB
Not used for evaluationyes
Score (nominal)0
Publication indicators Scopus Citations = 2; WoS Citations = 0; GS Citations = 1.0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.64; WoS Impact Factor: 2018 = 1.074 (2) - 2018=1.249 (5)
Citation count*1 (2020-07-30)
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