Spraying of cell colloids in medical atomizers

Tomasz Robert Sosnowski , Anita Kurowska , Beata Aleksandra Butruk , Katarzyna Jabłczyńska

Abstract

Aerosols are conveniently used for delivery of medicines to the human body. The most common application is inhalation of anti-asthmatic or anti-inflammatory drugs, nevertheless the spectrum of therapeutic agents that may be administered via the respiratory system is continuously expanding. Aerosols are also used topically (on skin) as a method of speeding up the healing. In this context, biocolloids (e.g., cell suspensions) are considered as the material for which the proper atomization method must be established to assure that local hydrodynamic stresses related to droplet formation will not destroy the living cells. In this work we test the influence of selected spraying techniques on the integrity and survival of different types of cells after the atomization process using medical atomizers. Medical nebulizers and spray devices were characterized in respect of emitted droplet size distribution and used to aerosolize model bio-colloids (suspensions of yeast, bacteria and mouse fibroblasts). Cell viability after spraying was determined via direct microscopic observations and specific microbiological assays. Commonly used medical inhalers produce droplets which are too small (Dv50 ≈ 4-5.5 μm) to contain whole living cells. As generation of such fine droplets is related to high local hydrodynamic stresses, cell viability in the suspension is strongly reduced even for small and mechanically resistant cells. Nasal atomizer (spray pump) and mechnical Microsprayer produce larger droplets (Dv50 ≈ 50-80 ��m) which are capable of carrying intact fibroblast cells. Due to a lower shear during atomization process in these devices, the cells remain viable and active. Based on the results of experimental study, only selected techniques can be proposed for bio-colloid atomization in medical applications.
Author Tomasz Robert Sosnowski (FCPE / CIPE)
Tomasz Robert Sosnowski,,
- Chair of Integrated Process Engineering
, Anita Kurowska (FCPE)
Anita Kurowska,,
- Faculty of Chemical and Process Engineering
, Beata Aleksandra Butruk (FCPE / DBBE)
Beata Aleksandra Butruk,,
- Department of Biotechnology and Bioprocess Engineering
, Katarzyna Jabłczyńska (FCPE / CIPE)
Katarzyna Jabłczyńska,,
- Chair of Integrated Process Engineering
Journal seriesChemical Engineering Transactions, ISSN 1974-9791
Issue year2013
Vol32
Pages2257-2262
Publication size in sheets0.5
ASJC Classification1500 General Chemical Engineering
DOIDOI:10.3303/CET1332377
URL http://www.aidic.it/cet/13/32/377.pdf
Languageen angielski
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Sosnowski T.R. (i in.) - Spraying of cell coloids....pdf 438.63 KB
Score (nominal)0
Score sourcejournalList
ScoreMinisterial score = 0.0, 27-04-2020, ArticleFromJournal
Ministerial score (2013-2016) = 0.0, 27-04-2020, ArticleFromJournal
Publication indicators WoS Citations = 9; Scopus Citations = 10; GS Citations = 15.0; Scopus SNIP (Source Normalised Impact per Paper): 2013 = 0.823
Citation count*15 (2020-09-20)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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