A label-free electrochemical DNA aptasensor for the detection of dopamine

Marta Maria Jarczewska , Sravanthi Reddy Sheelam , Robert Ziółkowski , Łukasz Górski

Abstract

Application of aptamers as the sensing layers in biosensors offers several advantages for the detection of various analytes. Herein, an electrochemical label - free biosensor for dopamine (DA) was developed with the use of 57-mer DNA aptamer. The proposed aptasensor operates via specific interaction between dopamine and aptamer sequence tethered to the gold disk electrode via thiol group. DA concentration was determined without use of any external redox indicator and the dopamine oxidation current serves as the analytical signal. Electrodes modified with DNA aptamer show selectivity toward dopamine within micromolar range with the lower detection limit of 26.8 μmol · L-1. The developed assay exhibits minor response in the presence of various interferents and its utility was tested in sample of diluted serum. Furthermore, the performance of proposed sensor was improved with LOD of 3.36 μmol · L-1 and the range of linear response from 5 to 75 μmol · L-1 by utilization of DNA aptamer/AuNP/reduced graphene oxide - modified glassy carbon electrodeApplication of aptamers as the sensing layers in biosensors offers several advantages for the detection of various analytes. Herein, an electrochemical label - free biosensor for dopamine (DA) was developed with the use of 57-mer DNA aptamer. The proposed aptasensor operates via specific interaction between dopamine and aptamer sequence tethered to the gold disk electrode via thiol group. DA concentration was determined without use of any external redox indicator and the dopamine oxidation current serves as the analytical signal. Electrodes modified with DNA aptamer show selectivity toward dopamine within micromolar range with the lower detection limit of 26.8 μmol · L-1. The developed assay exhibits minor response in the presence of various interferents and its utility was tested in sample of diluted serum. Furthermore, the performance of proposed sensor was improved with LOD of 3.36 μmol · L-1 and the range of linear response from 5 to 75 μmol · L-1 by utilization of DNA aptamer/AuNP/reduced graphene oxide - modified glassy carbon electrode.
Author Marta Maria Jarczewska ZMB
Marta Maria Jarczewska,,
- Department Of Microbioanalytics
, Sravanthi Reddy Sheelam
Sravanthi Reddy Sheelam,,
-
, Robert Ziółkowski ZMB
Robert Ziółkowski,,
- Department Of Microbioanalytics
, Łukasz Górski ZMB
Łukasz Górski,,
- Department Of Microbioanalytics
Journal seriesJournal of the Electrochemical Society, ISSN 0013-4651
Issue year2016
Vol163
No3
PagesB26-B31
Publication size in sheets0.5
Keywords in Englishaptasensor; dopamine determination; impedance spectroscopy; reduced graphene oxide; self-assembled monolayer; voltammetry
DOIDOI:10.1149/2.0501603jes
URL http://jes.ecsdl.org/content/163/3/B26
Languageen angielski
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J. Electrochem. Soc.-2016-Jarczewska-B26-31.pdf (file archived - login or check accessibility on faculty) J. Electrochem. Soc.-2016-Jarczewska-B26-31.pdf 446.39 KB
Score (nominal)40
ScoreMinisterial score = 40.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.259 (2) - 2016=3.307 (5)
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