Clinical significance of nucleoli cytomorphology assessment in patients with uveal melanoma

Tomasz Berus , Anna Markiewicz , Przemysław Biecek , Jolanta Orłowska-HEITZMAN , Agnieszka Hałoń , Bozena Romanowska-Dixon , Piotr Donizy

Abstract

Aim: To assess the prognostic significance of nucleolar morphological parameters in a large cohort of patients with uveal melanoma. Patients and Methods: The presence, size and number of nucleoli of cancer cells were assessed in haematoxylin and eosin (HE)-stained slides of 164 formalin-fixed paraffin-embedded primary uveal melanoma tissue specimens. The results were correlated with clinicopathological features and patient survival. Results: The presence of macronucleoli and multiple nucleoli significantly correlated with the epithelioid type of uveal melanoma, high mitotic rate, and marked pleomorphism. There was a positive correlation between the presence of macronucleoli as well as the number of nucleoli and the largest tumour basal diameter. The increased nucleolus count in tumour cells positively correlated with primary tumour (pT) staging. The presence of both prominent and multiple nucleoli was associated with significantly reduced overall and disease-free survival. Conclusion: Histological assessment of nucleolar morphology in routine HE staining would be a helpful low-cost method to obtain reliable prognostic information.

Author Tomasz Berus - [4th Military Clinical Hospital, Wroclaw]
Tomasz Berus,,
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, Anna Markiewicz - [Uniwersytet Jagielloński Collegium Medicum]
Anna Markiewicz,,
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, Przemysław Biecek (FMIS / DCSDCAM)
Przemysław Biecek,,
- Department of CAD/CAM Systems Design and Computer-Aided Medicine
, Jolanta Orłowska-HEITZMAN - [Uniwersytet Jagielloński Collegium Medicum]
Jolanta Orłowska-HEITZMAN,,
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, Agnieszka Hałoń - [Wroclaw Medical University]
Agnieszka Hałoń,,
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, Bozena Romanowska-Dixon - [Uniwersytet Jagielloński Collegium Medicum]
Bozena Romanowska-Dixon,,
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, Piotr Donizy - [Wroclaw Medical University]
Piotr Donizy,,
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Journal seriesAnticancer Research, ISSN 0250-7005, e-ISSN 1791-7530
Issue year2020
Vol40
No6
Pages3505-3512
Publication size in sheets0.5
ASJC Classification2700 General Medicine; 2730 Oncology; 1306 Cancer Research
DOIDOI:10.2196/10.21873/anticanres.14338
URL http://ar.iiarjournals.org/content/40/6/3505.abstract
Languageen angielski
Score (nominal)70
Score sourcejournalList
ScoreMinisterial score = 70.0, 11-08-2020, ArticleFromJournal
Publication indicators Scopus Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.637; WoS Impact Factor: 2018 = 1.935 (2) - 2018=1.883 (5)
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