Selection of methods for activated sludge bulking control using a molecular biology technique combined with respirometric tests

Aleksandra Miłobędzka , Adam Muszyński


An excessive proliferation of certain groups of filamentous bacteria results in activated sludge (AS) bulking and foaming. Studies on the efficiency of specific and non-specific methods for AS bulking control were conducted. Polyaluminium compounds (PAX16 and PAX18) were used as specific methods, whereas changes in pH and in temperature were tested among non-specific methods. Floatation of AS was determined by the Alka-Seltzer-test, ability of AS to settle was measured as sludge volume index. Oxygen uptake rate tests were carried out to determine respiratory activity of AS. Fluorescence in situ hybridisation was applied to identify and quantify filamentous bacteria in AS, which cannot be obtained by the conventional method. Two dominant groups of filamentous bacteria – phylum Chloroflexi and genus Microthrix were found in AS samples, the latter was the causative agent for bulking. The nonspecific methods for sludge bulking control (pH/temperature changes) improved the settling properties, however they did not eliminate filamentous bacteria and in some cases decreased sludge respiratory activity. PAX16 (5.7 mg/kg SS) was the most efficient control strategy for bulking caused by Microthrix – it improved settling properties without increasing tendency to foam and did not decrease respiratory activity of AS.
Author Aleksandra Miłobędzka (FEE / DB)
Aleksandra Miłobędzka,,
- Department of Biology
, Adam Muszyński (FEE / DB)
Adam Muszyński,,
- Department of Biology
Journal seriesBioTechnologia Journal of Biotechnology, Computational Biology and Bionanotechnology, ISSN 0860-7796, e-ISSN 2353-9461
Issue year2016
Publication size in sheets0.5
Keywords in EnglishOUR, FISH, filamentous bacteria, Microthrix, Chloroflexi
ASJC Classification1110 Plant Science; 1305 Biotechnology
Languageen angielski
Score (nominal)13
Score sourcejournalList
ScoreMinisterial score = 13.0, 25-03-2020, ArticleFromJournal
Ministerial score (2013-2016) = 13.0, 25-03-2020, ArticleFromJournal
Publication indicators Scopus Citations = 1; GS Citations = 2.0; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 0.302
Citation count*2 (2020-09-12)
Share Share

Get link to the record

* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Are you sure?