Back
Potential interactions between the TBX4-FGF10 and SHH-FOXF1 signaling during human lung development revealed using ChIP-seq
Authors:
- Justyna A. Karolak,
- Tomasz Gambin,
- Przemyslaw Szafranski,
- P. Stankiewicz
- Record ID
- WUTb3b404aa38f24fec878c0a78970d0fb0
- Author
- Journal series
- Respiratory Research, ISSN 1465-993X, e-ISSN 1465-9921, [1465-9921]
- Issue year
- 2021
- Vol
- 22
- No
- 1
- Pages
- 1-15
- ASJC Classification
- Abstract in original language
- Background:The epithelial-mesenchymal signaling involving SHH-FOXF1, TBX4-FGF10, and TBX2 pathways is an essential transcriptional network operating during early lung organogenesis. However, precise regulatory interactions between different genes and proteins in this pathway are incompletely understood.Methods:To identify TBX2 and TBX4 genome-wide binding sites, we performed chromatin immunoprecipitation followed by next-generation sequencing (ChIP-seq) in human fetal lung fibroblasts IMR-90.Results:We identified 14,322 and 1,862 sites strongly-enriched for binding of TBX2 and TBX4, respectively, 43.95% and 18.79% of which are located in the gene promoter regions. Gene Ontology, pathway enrichment, and DNA bind-ing motif analyses revealed a number of overrepresented cues and transcription factor binding motifs relevant for lung branching that can be transcriptionally regulated by TBX2 and/or TBX4. In addition, TBX2 and TBX4 binding sites were found enriched around and within FOXF1 and its antisense long noncoding RNA FENDRR, indicating that the TBX4-FGF10 cascade may directly interact with the SHH-FOXF1 signaling.Conclusions:We highlight the complexity of transcriptional network driven by TBX2 and TBX4 and show that disrup-tion of this crosstalk during morphogenesis can play a substantial role in etiology of lung developmental disorders.Keywords:Transcriptional regulation, Motif enrichment, Lung morphogenesis
- DOI
- DOI:10.1186/s12931-021-01617-y Opening in a new tab
- URL
- https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-021-01617-y Opening in a new tab
- Language
- eng (en) English
- License
- File
-
- File: 1
- Potential interactions between the TBX4-FGF10 and SHH-FOXF1 signaling during human lung development revealed using ChIP-seq, File WUTb3b404aa38f24fec878c0a78970d0fb0.pdf / 4 MB
- WUTb3b404aa38f24fec878c0a78970d0fb0.pdf
- publication date: 24-01-2022
- Potential interactions between the TBX4-FGF10 and SHH-FOXF1 signaling during human lung development revealed using ChIP-seq, File WUTb3b404aa38f24fec878c0a78970d0fb0.pdf / 4 MB
-
- Score (nominal)
- 100
- Score source
- journalList
- Score
- = 100.0, 08-05-2022, ArticleFromJournal
- Publication indicators
- = 4; = 1; = 1; : 2017 = 1.154; : 2018 (2 years) = 3.829 - 2018 (5 years) =4.184
- Citation count
- 4
- Uniform Resource Identifier
- https://repo.pw.edu.pl/info/article/WUTb3b404aa38f24fec878c0a78970d0fb0/
- URN
urn:pw-repo:WUTb3b404aa38f24fec878c0a78970d0fb0
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or PerishOpening in a new tab system.