Digital Holographic Microscopy with extended field of view using tool for generic image stitching

Piotr Stępień , Damian Korbuszewski , Małgorzata Kujawińska


This paper describes in detail the processing path that leads to successful phase-image stitching in digital holographic microscopy for the extension of the field of view. It applies the FIJI Grid/Collection Stitching Plugin, which is a general tool for image stitching that is not specific to phase images. The FIJI plugin is extensively supported by aberration and phase-offset correction. A comparative analysis of different aberration-correction methods and data-processing strategies is presented, together with a critical analysis of their applicability. The proposed processing path provides a good background for statistical phase analysis of cell cultures and digital phase pathology.
Author Piotr Stępień (FM / IMPh)
Piotr Stępień,,
- The Institute of Micromechanics and Photonics
, Damian Korbuszewski - [Politechnika Warszawska]
Damian Korbuszewski,,
, Małgorzata Kujawińska (FM / IMPh)
Małgorzata Kujawińska,,
- The Institute of Micromechanics and Photonics
Journal seriesETRI Journal, ISSN 1225-6463, e-ISSN 2233-7326, (N/A 40 pkt)
Issue year2019
Publication size in sheets0.5
Keywords in Englishdigital holography, digital holographic microscopy, aberration correction, stitching, cell culture analysis
ASJC Classification2208 Electrical and Electronic Engineering; 1700 General Computer Science; 2504 Electronic, Optical and Magnetic Materials
ProjectTEAM TECH BiOpTo: Tomographic phase microscope for biomedical applications. Project leader: Kujawińska Małgorzata, , Phone: +48 22234-8489, start date 01-10-2016, planned end date 31-10-2019, 503/00093, Implemented
WM Program Operacyjny Inteligentny Rozwój (PO IR)
Languageen angielski
Score (nominal)40
Score sourcejournalList
ScoreMinisterial score = 40.0, 20-10-2019, ArticleFromJournal
Publication indicators Scopus Citations = 0; WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 0.947; WoS Impact Factor: 2017 = 0.739 (2) - 2017=0.722 (5)
Citation count*1 (2019-11-18)
Additional fields
Special issue“Digital Holographic 3D Imaging: Capture, Display, and Evaluation”
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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