On the Interaction between Digitonin and Cholesterol in Langmuir Monolayers

Kamil Wojciechowski , Marta Orczyk , Thomas Gutberlet , Gerald Brezesinski , Thomas Geue , Philippe Fontaine

Abstract

In this article, we describe the effect of a highly hemolytic saponin, digitonin, on model lipids cholesterol and dipalmitoylphosphatidylcholine (DPPC) using a combination of tensiometric (surface pressure and dilatational surface elasticity), spectroscopic (infrared reflection absorption spectroscopy, IRRAS), microscopic (fluorescence microscopy), and scattering techniques (neutron reflectivity, NR, and grazing incidence X-ray diffraction, GIXD). The monolayers of individual lipids and their 10:9 (mol/mol) mixture were exposed to an aqueous solution of digitonin (10–4 M) by subphase exchange using a setup developed recently in our laboratory. The results confirm that digitonin can adsorb onto both bare and lipid-covered water–air interfaces. In the case of DPPC, a relatively weak interaction can be observed, but the presence of cholesterol drastically enhances the effect of digitonin. The latter is shown to dissociate the weak cholesterol–DPPC complexes and to bind cholesterol in an additional layer attached to the original lipid monolayer.
Author Kamil Wojciechowski (FC / IBC / DM)
Kamil Wojciechowski,,
- Department Of Microbioanalytics
, Marta Orczyk (FC / IBC / DM)
Marta Orczyk,,
- Department Of Microbioanalytics
, Thomas Gutberlet
Thomas Gutberlet,,
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, Gerald Brezesinski
Gerald Brezesinski,,
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, Thomas Geue
Thomas Geue,,
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, Philippe Fontaine
Philippe Fontaine,,
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Journal seriesLangmuir, ISSN 0743-7463
Issue year2016
Vol32
No35
Pages9064-9073
Publication size in sheets0.5
ASJC Classification1603 Electrochemistry; 1607 Spectroscopy; 3110 Surfaces and Interfaces; 3104 Condensed Matter Physics; 2500 General Materials Science
DOIDOI:10.1021/acs.langmuir.6b01737
URL http://pubs.acs.org/doi/abs/10.1021/acs.langmuir.6b01737
Languageen angielski
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On the Interaction between Digitonin and Cholesterol in Langmuir Monolayers.pdf 515.86 KB
Score (nominal)35
Score sourcejournalList
ScoreMinisterial score = 35.0, 01-01-2020, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 01-01-2020, ArticleFromJournal
Publication indicators WoS Citations = 9; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.188; WoS Impact Factor: 2016 = 3.833 (2) - 2016=4.205 (5)
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