On the Interaction between Digitonin and Cholesterol in Langmuir Monolayers

Kamil Wojciechowski , Marta Orczyk , Thomas Gutberlet , Gerald Brezesinski , Thomas Geue , Philippe Fontaine


In this article, we describe the effect of a highly hemolytic saponin, digitonin, on model lipids cholesterol and dipalmitoylphosphatidylcholine (DPPC) using a combination of tensiometric (surface pressure and dilatational surface elasticity), spectroscopic (infrared reflection absorption spectroscopy, IRRAS), microscopic (fluorescence microscopy), and scattering techniques (neutron reflectivity, NR, and grazing incidence X-ray diffraction, GIXD). The monolayers of individual lipids and their 10:9 (mol/mol) mixture were exposed to an aqueous solution of digitonin (10–4 M) by subphase exchange using a setup developed recently in our laboratory. The results confirm that digitonin can adsorb onto both bare and lipid-covered water–air interfaces. In the case of DPPC, a relatively weak interaction can be observed, but the presence of cholesterol drastically enhances the effect of digitonin. The latter is shown to dissociate the weak cholesterol–DPPC complexes and to bind cholesterol in an additional layer attached to the original lipid monolayer.
Author Kamil Wojciechowski ZMB
Kamil Wojciechowski,,
- Department Of Microbioanalytics
, Marta Orczyk ZMB
Marta Orczyk,,
- Department Of Microbioanalytics
, Thomas Gutberlet
Thomas Gutberlet,,
, Gerald Brezesinski
Gerald Brezesinski,,
, Thomas Geue
Thomas Geue,,
, Philippe Fontaine
Philippe Fontaine,,
Journal seriesLangmuir, ISSN 0743-7463
Issue year2016
Publication size in sheets0.5
URL http://pubs.acs.org/doi/abs/10.1021/acs.langmuir.6b01737
Languageen angielski
On the Interaction between Digitonin and Cholesterol in Langmuir Monolayers.pdf 515.86 KB
Score (nominal)35
ScoreMinisterial score = 35.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.833 (2) - 2016=4.205 (5)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.