Enhanced bioproduction of 2-phenylethanol in a biphasic system with rapeseed oil

Karolina Chreptowicz , Jolanta Mierzejewska


Increasing demand for natural fragrance ingredients and products is responsible for their global market growth. 2-Phenylethanol (2-PE) is a volatile substance widely used in food and cosmetics manufacturing. It is generally known that yeast can metabolize L-phenylalanine (L-Phe) to produce 2-PE. However, because the product exhibits an inhibitory effect on yeast cells, simple batch cultivation is uneconomic. The aim of this study was to enhance 2-PE productivity using in situ product removal. Here we present a new method of 2-PE production by yeast in a biphasic system with rapeseed oil as the second phase. The chosen solvent is safe, inexpensive and suitable for the extraction of 2-PE. In addition, rapeseed oil appeared to be a valuable source of intermediates for 2-PE synthesis as its presence in the yeast culture significantly enhanced productivity. The process is an environmentally friendly route and gives two final products that can be considered natural: rapeseed oil with a rose odor and pure 2-PE. Both may be subsequently used as food or cosmetic additives. The results obtained are competitive with previously reported values, as it was possible to enhance the overall concentration of 2-PE by 2.7-fold. The total 2-PE concentration in the biphasic system in the 4.5-L biofermentor used was increased to 9.79 g/L, while the 2-PE concentration in the organic phase attained a value of 18.50 g/L.
Author Karolina Chreptowicz (FC / IBC / DDTB)
Karolina Chreptowicz,,
- Department Of Drug Technology And Biotechnology
, Jolanta Mierzejewska (FC / IBC / DDTB)
Jolanta Mierzejewska,,
- Department Of Drug Technology And Biotechnology
Journal seriesNew Biotechnology, ISSN 1871-6784, (A 30 pkt)
Issue year2018
Publication size in sheets0.5
Keywords in English2-phenylethanol; Saccharomyces cerevisiae; in situ product removal; extraction; rapeseed oil
ASJC Classification1312 Molecular Biology; 2700 General Medicine; 1502 Bioengineering; 1305 Biotechnology
URL https://www.ncbi.nlm.nih.gov/pubmed/29476815
Languageen angielski
1-s2.0-S1871678417301097-main.pdf 432.53 KB
Score (nominal)30
Score sourcejournalList
ScoreMinisterial score = 30.0, 02-02-2020, ArticleFromJournal
Publication indicators Scopus Citations = 7; WoS Citations = 5; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.269; WoS Impact Factor: 2018 = 3.739 (2) - 2018=3.949 (5)
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