Uncommon and parallel developmental patterns of thymidylate synthase expression and localization in Trichinella spiralis and Caenorhabditis elegans

Magdalena Dąbrowska , Barbara Gołos , Elżbieta Wałajtys-Rode , Zbigniew Zieliński , Patrycja Wińska , Joanna Cieśla , Tadeusz Moczoń , Wojciech Rode

Abstract

Trichinella spiralis is a parasitic nematode causing trichinellosis, a serious disease, and Caenorhabditis elegans is a free-living nematode, which is used as a model in parasitological studies. High levels of thymidylate synthase (EC 2.1.1.45; ThyA) and certain other enzymes involved in thymidylate biosynthesis were found throughout T. spiralis and C. elegans developmental cycles, including developmentally arrested forms, that is, T. spiralis muscle larva and C. elegans dauer larva. As ThyA activity is characteristic for cells that left the G0 phase of the cell cycle, an exceptional regulation of the cell cycle in nematodes is suggested, manifested by a global cell cycle arrest in developmentally arrested larvae of the two species. ThyA immunolocalization during development of T. spiralis and C. elegans revealed the presence of high enzyme levels not only in the developing embryos, where it was expected, but also in gonad primordia, egg and sperm cells, nerve ring and secretory cells, opening to T. spiralis esophagus and C. elegans pharynx, where it may point to those cell populations remaining cell cycle arrested.
Author Magdalena Dąbrowska - [Nencki Institute of Experimental Biology of the Polish Academy of Sciences]
Magdalena Dąbrowska,,
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, Barbara Gołos - [Nencki Institute of Experimental Biology of the Polish Academy of Sciences]
Barbara Gołos,,
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, Elżbieta Wałajtys-Rode (FC / IBC / DDTB)
Elżbieta Wałajtys-Rode,,
- Department Of Drug Technology And Biotechnology
, Zbigniew Zieliński - [Nencki Institute of Experimental Biology of the Polish Academy of Sciences]
Zbigniew Zieliński,,
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, Patrycja Wińska
Patrycja Wińska,,
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, Joanna Cieśla - [Nencki Institute of Experimental Biology PAS, Warsaw]
Joanna Cieśla,,
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- Nencki Institute of Experimental Biology PAS, Warsaw
, Tadeusz Moczoń - [Witold Stefanski Institute of Parasitology of the Polish Academy of Sciences]
Tadeusz Moczoń,,
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, Wojciech Rode - [Nencki Institute of Experimental Biology of the Polish Academy of Sciences]
Wojciech Rode,,
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Journal seriesPteridines, ISSN 0933-4807
Issue year2013
Vol24
No1
Pages121-125
Publication size in sheets0.5
Keywords in EnglishCaenorhabditis elegans, thymidylate synthase, Trichinella spiralis, Enzymes: thymidylate synthase (EC 2.1.1.45), dihydrofolate reductase (EC 1.5.1.3), dUTP-ase (EC 3.6.1.23), ribonucleotide reductase (EC 1.17.4.1)
ASJC Classification1308 Clinical Biochemistry; 1313 Molecular Medicine; 1303 Biochemistry
DOIDOI:10.1515/pterid-2013-0009
URL http://www.degruyter.com/view/j/pteridines.2013.24.issue-1/pterid-2013-0009/pterid-2013-0009.xml
Languageen angielski
File
wdpb_publikacje_pliki_plik_publikacja_2039_org.pdf 902.92 KB
Score (nominal)15
Score sourcejournalList
ScoreMinisterial score = 15.0, 02-02-2020, ArticleFromJournal
Ministerial score (2013-2016) = 15.0, 02-02-2020, ArticleFromJournal
Publication indicators Scopus Citations = 0; WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2014 = 0.209; WoS Impact Factor: 2013 = 0.963 (2) - 2013=0.57 (5)
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