Electrolyte alginate/poly-l-lysine membranes for connective tissue development

Alicja Kosik , Urszula Luchowska , Wojciech Święszkowski

Abstract

The aim of this study was to change the surface of sodium alginate hydrogel by electrostatic binding of poly-l-lysine (PLL) in order to provide more advantageous conditions for connective tissue development. Its impact on L929 mouse fibroblast adhesion, morphology and viability was investigated. Analysis of the material microstructure has shown that performed modification increased surface roughness. It also altered the swelling properties of alginate hydrogel, resulting in less rapid water absorption. Mouse fibroblasts seeded on regular alginate and alginate modified with PLL exhibited different behaviour. The presence of PLL turned out to promote cell adhesion and F-actin spreading, resulting in significantly increased number of viable cells 96 h after seeding.
Author Alicja Kosik ZPM
Alicja Kosik,,
- Division of Materials Design
, Urszula Luchowska ZPM
Urszula Luchowska,,
- Division of Materials Design
, Wojciech Święszkowski ZPM
Wojciech Święszkowski,,
- Division of Materials Design
Journal seriesMaterials Letters, ISSN 0167-577X
Issue year2016
Vol184
Pages104-107
Publication size in sheets0.5
Keywords in EnglishAlginate; Poly-l-lysine; Electrolyte membranes; Cell adhesion
DOIDOI:10.1016/j.matlet.2016.08.032
URL http://www.sciencedirect.com/science/article/pii/S0167577X16313118
Languageen angielski
Score (nominal)35
ScoreMinisterial score = 35.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.572 (2) - 2016=2.426 (5)
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