Electrochemical aptamer-based biosensors as potential tools for clinical diagnostics

Marta Maria Jarczewska , Łukasz Górski , Elżbieta Malinowska

Abstract

Aptamers are single-stranded DNA and RNA sequences that belong to the group of "functional nucleic acids", which exhibit both catalytic and receptor properties. Aptamers change their spatial conformation upon binding to a target analyte, and so far more than 200 sequences selective for large variety of molecules such as metal ions, organic dyes, proteins and bacteria cells have been identified. Aptamers can be applied in several fields including diagnostics, therapy and as the recognition layers for biosensors, which is one of the most promising areas. The utilization of aptamers as receptor elements in electrochemical assays requires not only the choice of an appropriate immobilization method with respect to the detected target, but also experimental conditions including the manner of analytical signal generation. The latter issue might affect the efficiency of binding between the aptamer-modified surface and analyte, and consequently the sensitivity of target molecule quantification. Herein, a review of electrochemical aptasensors dedicated to the determination of target analytes crucial in clinical diagnostics developed during the past 10 years is presented. It contains a short characterization of aptamers and their application as sensing layers in electrochemical assays, which is followed by a description of the examples of the use of aptasensors
Author Marta Maria Jarczewska ZMB
Marta Maria Jarczewska,,
- Department Of Microbioanalytics
, Łukasz Górski ZMB
Łukasz Górski,,
- Department Of Microbioanalytics
, Elżbieta Malinowska ZMB
Elżbieta Malinowska,,
- Department Of Microbioanalytics
Journal seriesAnalytical Methods, ISSN 1759-9660
Issue year2016
Vol8
No19
Pages3861-3877
Publication size in sheets0.8
DOIDOI:10.1039/c6ay00499g
URL http://pubs.rsc.org.doi.eczyt.bg.pw.edu.pl/en/Content/ArticleLanding/2016/AY/C6AY00499G#!divAbstract
Languageen angielski
File
c6ay00499g.pdf 1.03 MB
Score (nominal)25
ScoreMinisterial score [Punktacja MNiSW] = 25.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) [Punktacja MNiSW (2013-2016)] = 25.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor [Impact Factor WoS]: 2016 = 1.9 (2) - 2016=1.893 (5)
Citation count*0
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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