Increase of radiopacity of PCL scaffolds for their in vivo monitoring using x – rays imaging
Żaneta Górecka , Emilia Choińska , Karol Szlązak , Wojciech Święszkowski
AbstractINTRODUCTION: In vitro studies and mathematical modeling are very helpful in predicting behaviour of scaffolds after implantation. However, they cannot substitute in vivo monitoring. For this purpose, scaffolds should be visible in X-rays that enables to observe changes in morphology, detect cracks and defects, etc.  Contrast properties of polyesters can be improved by adding some radiopaque fillers.  The aim of the study was to investigate the influence of radiopaque filler barium sulfate (BaSO4) on widely used polycaprolactone–hydroxyapatite (PCL-HAp) composites. METHODS: Materials used in following experiments were prepared by solvent casting technique, dried in air at room temperature and then in a vacuum dryer. The compositions of prepared materials are presented in Table 1. Rods made of pure polymer and composites were fabricated by Fused Deposition Modeling (FDM) method and then incubated in phosphate-buffered saline (PBS). Calculations of mass loss and water absorption, micro computed tomography and cytotoxicity assay were used to characterise materials during degradation. RESULTS: The obtained composite rods had different image intensity. BaSO4 caused strong increase of visibility using X-rays but not increase of degradation rate of polymeric matrix. Additionally, higher content of HAp caused higher degradation rate and also increase of radiopaque properties of composite. There was no cytotoxic effect of using a BaSO4 as a filler in contact with murine fibroblast cell line L929. DISCUSSION & CONCLUSIONS: The concept of using a BaSO4 as a filler in scaffold material allows for a non-invasive approach for in vivo imaging. Absence of serious hazards in presence of BaSO4 was also observed in other works. [3,4] The results confirmed there is a possibility to obtain non-cytotoxic scaffold composition which enables monitoring of its stability after implantation in surrounding soft tissue, even in bone tissue.
|Journal series||European Cells & Materials, ISSN 1473-2262|
|Publication size in sheets||0.3|
|ASJC Classification||; ; ; ;|
|Publication indicators||: 2016 = 1.28; : 2016 = 3.343 (2) - 2016=4.803 (5)|
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