Application of DNA aptamers as sensing layers for electrochemicaldetection of potassium ions

Marta Maria Jarczewska , Łukasz Górski , Elżbieta Malinowska

Abstract

Determination of potassium level in organism is of special importance due to its vital role in the maintenance of physiological activities in organism. Herein, an electrochemical sensor for K+ ions was developed with recognition layer based on DNA aptamers. The elaborated assay was formed by tethering of thiolated aptamer probes to gold disk electrode via Au-S bond. Potassium concentration was determined with the use of voltammetric techniques by comparison of current change of redox indicators—methylene blue (MB), AQMS and Na4Fe(CN)6. The studies revealed that among aptamer sequences, which were previously shown to bind with potassium ions, 15-mer thrombin binding aptamer (TBA) exhibited highest affinity towards potassium with Kd = 1.13 μmol L−1 when used as a sensing layer. The aptasensor demonstrated linear response within the range from 10−8 to 10−5 mol L−1 with a LOD of 2.31 × 10−9 mol L−1 and good selectivity towards K+ ions with the use of MB electroactive marker.
Author Marta Maria Jarczewska ZMB
Marta Maria Jarczewska,,
- Department Of Microbioanalytics
, Łukasz Górski ZMB
Łukasz Górski,,
- Department Of Microbioanalytics
, Elżbieta Malinowska ZMB
Elżbieta Malinowska,,
- Department Of Microbioanalytics
Journal seriesSensors and Actuators B - Chemical, ISSN 0925-4005
Issue year2016
Vol226
Pages37-43
Publication size in sheets0.5
Keywords in EnglishPotassium detection; Self-assembled monolayers; Aptasensor; Redox indicators; Voltammetry
DOIDOI:10.1016/j.snb.2015.11.139
URL http://www.sciencedirect.com/science/article/pii/S0925400515307218
Languageen angielski
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Score (nominal)40
ScoreMinisterial score = 40.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 5.401 (2) - 2016=4.855 (5)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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