A bioactive hybrid three-dimensional tissue-engineering construct for cartilage repair

M. Ainola , W. Tomaszewski , Barbara Ostrowska , E. Wesołowska , H.D. Wagner , Wojciech Święszkowski , T. Sillat , E. Peltola , Y.T. Konttinen

Abstract

The aim was to develop a hybrid three-dimensional-tissue engineering construct for chondrogenesis. The hypothesis was that they support chondrogenesis. A biodegradable, highly porous polycaprolactone-grate was produced by solid freeform fabrication. The polycaprolactone support was coated with a chitosan/polyethylene oxide nanofibre sheet produced by electrospinning. Transforming growth factor-3-induced chondrogenesis was followed using the following markers: sex determining region Y/-box 9, runt-related transcription factor 2 and collagen II and X in quantitative real-time polymerase chain reaction, histology and immunostaining. A polycaprolactone-grate and an optimized chitosan/polyethylene oxide nanofibre sheet supported cellular aggregation, chondrogenesis and matrix formation. In tissue engineering constructs, the sheets were seeded first with mesenchymal stem cells and then piled up according to the lasagne principle. The advantages of such a construct are (1) the cells do not need to migrate to the tissue engineering construct and therefore pore size and interconnectivity problems are omitted and (2) the cell-tight nanofibre sheet and collagen-fibre network mimic a cell culture platform for mesenchymal stem cells/chondrocytes (preventing escape) and hinders in-growth of fibroblasts and fibrous scarring (preventing capture). This allows time for the slowly progressing, multiphase true cartilage regeneration.
Author M. Ainola
M. Ainola,,
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, W. Tomaszewski
W. Tomaszewski,,
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, Barbara Ostrowska ZPM
Barbara Ostrowska,,
- Division of Materials Design
, E. Wesołowska
E. Wesołowska,,
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, H.D. Wagner
H.D. Wagner,,
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, Wojciech Święszkowski ZPM
Wojciech Święszkowski,,
- Division of Materials Design
, T. Sillat
T. Sillat,,
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, E. Peltola
E. Peltola,,
-
, Y.T. Konttinen
Y.T. Konttinen,,
-
Journal seriesJournal of Biomaterials Applications, ISSN 0885-3282
Issue year2016
Vol30
No6
Pages873-885
Publication size in sheets0.6
Keywords in EnglishScaffold; cartilage; chondrogenesis; rapid prototyping; nanofibres; chitosan
DOIDOI:10.1177/0885328215604069
URL http://jba.sagepub.com/content/30/6/873
Languageen angielski
Score (nominal)30
ScoreMinisterial score = 25.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.31 (2) - 2016=2.346 (5)
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