Studies on influence of polymer modifiers for fluorescent nanocrystals' cytotoxicity

Ilona Grabowska-Jadach , Marcin Drozd , Joanna Biegała , Mariusz Pietrzak , Marta Mazurkiewicz-Pawlicka , Paweł Parzuchowski , Zbigniew Brzózka

Abstract

The presented studies aimed at investigation of the effect of CdSeS/ZnS quantum dots (QDs) stabilized with hyperbranched polyglycidol and its carboxylated derivative on adenocarcinomic human alveolar basal epithelial cells (A549). The first stage of studies concerned the modification of quantum dots with both types of the tested polymers with the use of pyridine as an intermediate agent. Subsequently, cytotoxic effect of the prepared nanoparticles was examined after various incubation time using MTT test (cell metabolic activity assay). Our studies revealed that CdSeS/ZnS with a diameter of 6nm, which were stabilized with hyperbranched polymers do not penetrate into cells, even after prolonged incubation time. Moreover, the cytotoxic effect of the tested QDs was observed over a range of tested concentrations (5-90μM of Cd(2+)). It was confirmed that tested nanoparticles had significant influence on cell culture viability. The examined cytotoxic effect of the tested quantum dots was dependent on the type of polymer applied and the experiments indicated, that the one bearing carboxylic moieties is more toxic to A549 cells.
Author Ilona Grabowska-Jadach (FC / IBC / DM)
Ilona Grabowska-Jadach,,
- Department Of Microbioanalytics
, Marcin Drozd (FC / IBC / DM)
Marcin Drozd,,
- Department Of Microbioanalytics
, Joanna Biegała (FC / IBC / DM)
Joanna Biegała,,
- Department Of Microbioanalytics
, Mariusz Pietrzak (FC / IBC / DM)
Mariusz Pietrzak,,
- Department Of Microbioanalytics
, Marta Mazurkiewicz-Pawlicka (FCPE / GL)
Marta Mazurkiewicz-Pawlicka,,
- WUT Grafen Laboratory
, Paweł Parzuchowski (FC / CPCT)
Paweł Parzuchowski,,
- Chair Of Polymer Chemistry And Technology
, Zbigniew Brzózka (FC / IBC / DM)
Zbigniew Brzózka,,
- Department Of Microbioanalytics
Journal seriesJournal of Pharmaceutical and Biomedical Analysis, ISSN 0731-7085, (A 35 pkt)
Issue year2016
Vol127
NoCurrent trends in bioanalysis - In Honour of Professor Roman Kaliszan
Pages193-201
Publication size in sheets0.5
Keywords in English Quantum dots; Cytotoxicity; Polymer coating; Hyperbranched polymer
ASJC Classification1308 Clinical Biochemistry; 1607 Spectroscopy; 3002 Drug Discovery; 3003 Pharmaceutical Science; 1602 Analytical Chemistry
DOIDOI:10.1016/j.jpba.2016.02.048
URL http://www.sciencedirect.com/science/article/pii/S0731708516301108
Languageen angielski
File
1-s2.0-S0731708516301108-main.pdf 4.85 MB
Score (nominal)35
ScoreMinisterial score = 35.0, 12-09-2019, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 12-09-2019, ArticleFromJournal
Publication indicators WoS Citations = 4; Scopus Citations = 4; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.364; WoS Impact Factor: 2016 = 3.255 (2) - 2016=2.953 (5)
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