N-doped carbon nanoparticles: properties and influence on mouse fibroblast L929 cells
- Piotr Wyrębiak,
- Aleksandra Poniatowska,
- Tomasz Ciach
Carbon dots (CDs), which are the newly discovered fluorescent nanomaterials, have become popular in the last decade due to their unique optical properties, good biocompatibility,possible low toxicity, high stability in water, and facility of synthesis. One of the types of CDs are nitrogen doped carbon nanoparticles (NCNPs). NCNPs have a non-zero bandwidth and with good fluorescent properties, can have much potential use in various fields. The aim of the research was to synthesize NCNPs from citric acid with nitrogen atoms incorporated in the structure of NCNPs and to study its effect on the viability of cells cultured in vitro. The nitrogen sources were urea, ammonia, ethanolamine, tris (2-amino- 2-(hydroxymethyl)propane-1,3-diol). Homogeneous, mixed solutions were pyrolyzed in 200 °C and various time variants: 1h, 1h 40 min, 2h 30 min, which is novelty to related articles. The analysis of the carbon nanoparticles physicochemical properties was investigated by Fourier transform infrared spectroscopy (FTIR), dynamic light scattering technique (particle size distribution), and fluorescence measurement. The acid-base characteristics were also examined. Because of the potential use in medicine, a commonly known short cytotoxicity test MTT was used for this purpose. The mouse fibroblasts cell line L929 was incubated at 37 °C for 24h with suspensions of nitrogen modified CNPs at a concentration of 1 mg/ml and 0.5 mg/ml. Particle size analysis showed that a wide distribution of diameter characterizes the obtained particles, especially NCNPs after synthesis with ammonia and urea. The highest relative fluorescence was obtained for carbon nanoparticles with nitrogen groups derived from Tris. The viability for cells incubated with this material was over 70%, so it is supposed that it has no negative influence on the viability of cells. On the contrary, NCNPs obtained with the addition of urea and ammonia turned out to be toxic to cells (viability below 40%). The research also shows differences between carbon-based materials with nitrogen depending on nitrogen sources and its properties are strongly dependent on the synthesis conditions and confirmed that carbon nanoparticles could be a promising material in medical applications,for example, cancer diagnostics or anti-cancer therapy.
- Record ID
- Article number
- EYEC Monograph: 9th European Young Engineers Conference, 2021, Warszawa, Politechnika Warszawska, Wydział Inżynierii Chemicznej i Procesowej, 183 p., ISBN 978-83-936575-9-9
- Keywords in English
- citric acid, nitrogen doped, carbon nanoparticles, fluorescence, cytotoxicity
- eng (en) English
- Score (nominal)
- Score source
- = 20.0, 09-05-2022, ChapterFromConference
- Uniform Resource Identifier
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