Insight into characteristic features of cartilage growth plate as a physiological template for bone formation

Jakub Jaroszewicz , A. Kosowska , Hutmacher Dietmar , Wojciech Święszkowski , W. Moskalewski

Abstract

Cartilage growth plate is a natural template from both a biochemical and structural point of view and allows osteoblasts migration, proliferation, differentiation, and ultimately, bone formation. It is evolutionary adjusted to support bone formation within strictly defined spatial framework serving as an interesting model for studying more mechanistically aspects which might be important for specific scaffold-based bone tissue engineering strategies. Surprisingly little is known about the geometric features of this physiological template. To this purpose we analyzed cartilage growth plate from rat, mouse, and human costochondral junction and tibia. High-resolution X-ray tomography showed that pore size in the zone of provisional calcification was within 20 to 30 mu m range and in the metaphysis in 35 to 50 mu m range. The thickness of calcified longitudinal septa in zone of provisional calcification was 3 to 5 mm and in metaphysis 7 to 12 mm. The porosity varied from 84 to 88%. We observed that numerical values characteristic for cartilage growth plate were not significantly influenced by the species of origin, by the type of bone, or by age. In addition, electron microscopy of calcified fragments of longitudinal septa showed that the calcium aggregates were globular, connected with each other, and formed a shell covering cartilage matrix located within longitudinal septa. (c) 2015 Wiley Periodicals, Inc.
Author Jakub Jaroszewicz WIM
Jakub Jaroszewicz,,
- Faculty of Materials Science and Engineering
, A. Kosowska
A. Kosowska,,
-
, Hutmacher Dietmar
Hutmacher Dietmar,,
-
, Wojciech Święszkowski ZPM
Wojciech Święszkowski,,
- Division of Materials Design
, W. Moskalewski
W. Moskalewski,,
-
Journal seriesJournal of Biomedical Materials Research Part A, ISSN 1549-3296 [1552-4965]
Issue year2016
Vol104
No2
Pages357-366
Publication size in sheets0.5
Keywords in Englishmicrotomography; cartilage growth plate; 3D scaffold; mineralization; calcium deposits
DOIDOI:10.1002/jbm.a.35575
URL http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.35575/abstract
Languageen angielski
Score (nominal)35
ScoreMinisterial score = 35.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.076 (2) - 2016=3.076 (5)
Citation count*1 (2016-09-12)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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