Application of RNA Aptamers as Recognition Layers for the Electrochemical Analysis of C-Reactive Protein

Marta Maria Jarczewska , Robert Ziółkowski , Łukasz Górski , Elżbieta Malinowska

Abstract

C-reactive protein (CRP) is one of the crucial biomarkers of inflammation, as well as cardiovascular and cancer diseases. Hence, the development of a sensitive and selective technique for CRP detection is of special importance. One of the possibilities is the application of biosensors containing receptor elements formed of RNA aptamers, as they exhibit high affinity towards C-reactive protein. Herein, the capability of utilization of thiolated RNA aptamers as sensing layers was verified. The occurrence of aptamer - C-reactive protein interaction was confirmed by quartz crystal microbalance experiments. The voltammetric measurements performed in the presence of methylene blue redox indicator showed a linear current decrease within 1–100 pmol L−1 range of protein concentration. The interaction between surface-tethered aptamer and CRP was distinguished with dissociation constant of 25.9 pmol L−1. The developed assay demonstrated a pronounced selectivity to CRP over interfering proteins as well as capability of CRP analysis in serum sample.
Author Marta Maria Jarczewska (FC / IBC / DM)
Marta Maria Jarczewska,,
- Department Of Microbioanalytics
, Robert Ziółkowski (FC / IBC / DM)
Robert Ziółkowski,,
- Department Of Microbioanalytics
, Łukasz Górski (FC / IBC / DM)
Łukasz Górski,,
- Department Of Microbioanalytics
, Elżbieta Malinowska (FC / IBC / DM)
Elżbieta Malinowska,,
- Department Of Microbioanalytics
Journal seriesElectroanalysis, ISSN 1040-0397, (A 30 pkt)
Issue year2018
Vol30
Pages658-664
Publication size in sheets0.5
Keywords in EnglishRNA aptamer, biosensor, voltammetry, methylene blue
ASJC Classification1603 Electrochemistry; 1602 Analytical Chemistry
DOIDOI:10.1002/elan.201700620
URL http://onlinelibrary.wiley.com/doi/10.1002/elan.201700620/full
Languageen angielski
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wdpb_publikacje_pliki_plik_publikacja_3320_org.pdf 2.36 MB
Score (nominal)30
ScoreMinisterial score = 30.0, 23-09-2019, ArticleFromJournal
Publication indicators WoS Citations = 1; Scopus Citations = 1; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 0.643; WoS Impact Factor: 2017 = 2.851 (2) - 2017=2.469 (5)
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