Effect of surfactants on surface activity and rheological properties of type I collagen at air/water interface

Aleksandra Kezwoń , Ilona Góral , Tomasz Frączyk , Kamil Wojciechowski


We describe the effect of three synthetic surfactants (anionic – sodium dodecyl sulfate (SDS), cationic – cetyltrimethylammonium bromide (CTAB) and nonionic – Triton X-100 (TX-100)) on surface properties of the type I calf skin collagen at the air/water interface in acidic solutions (pH 1.8). The protein concentration was fixed at 5 × 10−6 mol L−1 and the surfactant concentration was varied in the range 5 × 10−6 mol L−1–1 × 10−4 mol L−1, producing the protein/surfactant mixtures with molar ratios of 1:1, 1:2, 1:5, 1:10 and 1:20. An Axisymmetric Drop Shape Analysis (ADSA) method was used to determine the dynamic surface tension and surface dilatational moduli of the mixed adsorption layers. Two spectroscopic techniques: UV–vis spectroscopy and fluorimetry allowed us to determine the effect of the surfactants on the protein structure. The thermodynamic characteristic of the mixtures was studied using isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC). Modification of the collagen structure by SDS at low surfactant/protein ratios has a positive effect on the mixture’s surface activity with only minor deterioration of the rheological properties of the adsorbed layers. The collagen/CTAB mixtures do not show that pronounced improvement in surface activity, while rheological properties are significantly deteriorated. The mixtures with non-ionic TX-100 do not show any synergistic effects in surface activity.
Author Aleksandra Kezwoń ZMB
Aleksandra Kezwoń,,
- Department Of Microbioanalytics
, Ilona Góral ZMB
Ilona Góral,,
- Department Of Microbioanalytics
, Tomasz Frączyk
Tomasz Frączyk,,
, Kamil Wojciechowski ZMB
Kamil Wojciechowski,,
- Department Of Microbioanalytics
Journal seriesColloids and Surfaces B-Biointerfaces, ISSN 0927-7765
Issue year2016
Publication size in sheets0.5
Keywords in EnglishType I calf skin collagen; Surface tension; Surface dilatational rheology; Calorimetry; Spectrophotometry; Fluorimetry; SDS; CTAB; TRITON X-100
URL http://www.sciencedirect.com/science/article/pii/S092777651630635X
Languageen angielski
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Score (nominal)35
ScoreMinisterial score = 35.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.887 (2) - 2016=4.295 (5)
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