Gradients in pore size enhance the osteogenic differentiation of human mesenchymal stromal cells in three-dimensional scaffolds

A. Di Luca , Barbara Ostrowska , I. Lorenzo-Moldero , A. Lepedda , Wojciech Święszkowski , C. Van Blitterswijk , L. Moroni

Abstract

Small fractures in bone tissue can heal by themselves, but in case of larger defects current therapies are not completely successful due to several drawbacks. A possible strategy relies on the combination of additive manufactured polymeric scaffolds and human mesenchymal stromal cells (hMSCs). The architecture of bone tissue is characterized by a structural gradient. Long bones display a structural gradient in the radial direction, while flat bones in the axial direction. Such gradient presents a variation in bone density from the cancellous bone to the cortical bone. Therefore, scaffolds presenting a gradient in porosity could be ideal candidates to improve bone tissue regeneration. In this study, we present a construct with a discrete gradient in pore size and characterize its ability to further support the osteogenic differentiation of hMSCs. Furthermore, we studied the behaviour of hMSCs within the different compartments of the gradient scaffolds, showing a correlation between osteogenic differentiation and ECM mineralization, and pore dimensions. Alkaline phosphatase activity and calcium content increased with increasing pore dimensions. Our results indicate that designing structural porosity gradients may be an appealing strategy to support gradual osteogenic differentiation of adult stem cells.
Author A. Di Luca - [University of Twente (UT)
Administrator unit: 'COP'
]
A. Di Luca,,
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- Universiteit Twente
, Barbara Ostrowska (FMSE / DMD)
Barbara Ostrowska,,
- Division of Materials Design
, I. Lorenzo-Moldero - [Maastricht University]
I. Lorenzo-Moldero,,
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, A. Lepedda - [Università degli Studi di Sassari]
A. Lepedda,,
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, Wojciech Święszkowski (FMSE / DMD)
Wojciech Święszkowski,,
- Division of Materials Design
, C. Van Blitterswijk - [University of Twente]
C. Van Blitterswijk,,
-
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, L. Moroni - [University of Twente]
L. Moroni,,
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Journal seriesScientific Reports, ISSN 2045-2322
Issue year2016
Vol6
Pages1-13
Publication size in sheets0.6
ASJC Classification1000 Multidisciplinary
DOIDOI:10.1038/srep22898
URL https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4790631/
Languageen angielski
Score (nominal)40
Score sourcejournalList
ScoreMinisterial score = 40.0, 23-07-2020, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 23-07-2020, ArticleFromJournal
Publication indicators Scopus Citations = 68; WoS Citations = 29; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.401; WoS Impact Factor: 2016 = 4.259 (2) - 2016=4.847 (5)
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